Characterization of the cutaneous mycobiota in Persian cats with severe dermatophytosis.
Characterization of the cutaneous mycobiota in Persian cats with severe dermatophytosis.
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DOI：
10.1111/vde.12969
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发表时间：
2021-08
影响因子：
1.4
通讯作者：
Rodrigues Hoffmann A
中科院分区：
文献类型：
--
作者：
Myers AN;Older CE;Diesel AB;Lawhon SD;Rodrigues Hoffmann A
波斯猫容易患慢性和严重的皮肤癣菌病。皮肤微生物群的改变是导致这种倾向的一个潜在因素。表征患有慢性、严重皮肤癣菌病的波斯猫的皮肤和环境真菌微生物群，并比较患有和不患有皮肤癣菌病的猫的真菌微生物群。客户拥有的 36 只猫，其中包括 26 只波斯猫和 10 只家养长毛 (DLH) 猫。从患有严重慢性皮肤癣菌病的波斯猫以及健康对照猫（波斯猫和 DLH）中收集皮肤和家庭环境拭子。除了 ITS1 定量 PCR 和真菌培养之外，还对内部转录间隔区 1 (ITS1) 区域进行了测序。针对真菌 ITS 区域的新一代测序 (NGS) 检测到小孢子菌。来自所有被诊断患有皮肤癣菌病的波斯猫及其家中环境样本的 DNA。病例和对照之间的群落结构存在显着差异，这主要是由于小孢子菌所致。受影响猫样本中的 DNA。患有皮肤癣菌病的波斯猫并没有表现出真菌多样性下降。 NGS 未能在两个培养阳性的无症状波斯对照中鉴定出皮肤癣菌 DNA，并从培养阴性的无症状波斯对照中鉴定出红色毛癣菌 DNA。除了犬小孢子菌之外，我们的结果表明潜在的真菌失调不太可能在波斯猫皮肤癣菌病的发展中发挥作用。应该调查对这种疾病易感性的其他解释，例如原发性免疫缺陷、无效的梳理或波斯猫毛发的独特特征。
Persian cats are predisposed to chronic and severe dermatophytosis. Alterations to the cutaneous microbiota are one potential contributor to this predisposition. To characterise the cutaneous and environmental fungal microbiota of Persian cats with chronic, severe dermatophytosis, and to compare the fungal microbiota of cats with and without dermatophytosis. Thirty-six client-owned cats, including 26 Persian cats and 10 domestic long hair (DLH) cats. Skin and home environment swabs were collected from Persian cats with severe, chronic dermatophytosis as well as groups of healthy control cats (Persian and DLH). Sequencing of the internal transcribed spacer 1 (ITS1) region was performed in addition to ITS1 quantitative PCR and fungal culture. Next-generation sequencing (NGS) targeting the fungal ITS region detected Microsporum sp. DNA from all Persian cats diagnosed with dermatophytosis and from environmental samples of their homes. A significant difference in community structure was identified between cases and controls, largely due to the Microsporum sp. DNA in samples from affected cats. Persian cats with dermatophytosis do not exhibit decreased fungal diversity. NGS failed to identify dermatophyte DNA on two culture-positive asymptomatic Persian controls and identified Trichophyton rubrum DNA from a culture-negative asymptomatic Persian control. Aside from M. canis, our results indicate that an underlying fungal dysbiosis is not likely to play a role in development of dermatophytosis in Persian cats. Other explanations for predisposition to this disease, such as a primary immunodeficiency, ineffective grooming or unique features of Persian cat hair should be investigated.
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DOI：
10.1056/nejmoa1208487
发表时间：
2013-10-31
期刊：
The New England journal of medicine
影响因子：
--
作者：
Lanternier F;Pathan S;Vincent QB;Liu L;Cypowyj S;Prando C;Migaud M;Taibi L;Ammar-Khodja A;Stambouli OB;Guellil B;Jacobs F;Goffard JC;Schepers K;Del Marmol V;Boussofara L;Denguezli M;Larif M;Bachelez H;Michel L;Lefranc G;Hay R;Jouvion G;Chretien F;Fraitag S;Bougnoux ME;Boudia M;Abel L;Lortholary O;Casanova JL;Picard C;Grimbacher B;Puel A
通讯作者：
Puel A
影响因子：
3.5
作者：
Abraham A;Pedregosa F;Eickenberg M;Gervais P;Mueller A;Kossaifi J;Gramfort A;Thirion B;Varoquaux G
通讯作者：
Varoquaux G
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
15.5
作者：
Davis, Nicole M;Proctor, Diana M;Callahan, Benjamin J
通讯作者：
Callahan, Benjamin J
影响因子：
1.4
作者：
Nuttall, T. J.;German, A. J.;McEwan, N. A.
通讯作者：
McEwan, N. A.