The LRR-RLK Protein HSL3 Regulates Stomatal Closure and the Drought Stress Response by Modulating Hydrogen Peroxide Homeostasis.
The LRR-RLK Protein HSL3 Regulates Stomatal Closure and the Drought Stress Response by Modulating Hydrogen Peroxide Homeostasis.
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LRR-RLK 蛋白 HSL3 通过调节过氧化氢稳态来调节气孔关闭和干旱应激反应
DOI：
10.3389/fpls.2020.548034
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发表时间：
2020
影响因子：
5.6
通讯作者：
Wang M
中科院分区：
文献类型：
--
作者：
Liu XS;Liang CC;Hou SG;Wang X;Chen DH;Shen JL;Zhang W;Wang M
保卫细胞因干旱胁迫和脱落酸 (ABA) 信号而收缩，从而减小气孔孔径。过氧化氢 (H2O2) 是一种重要的信号分子，可诱导气孔关闭。迄今为止，控制保卫细胞中 H2O2 水平的分子基础仍然很大程度上未知。富含亮氨酸重复序列 (LRR) 的受体样激酶 (RLK) 蛋白 HSL3 已被证明能够通过调节保卫细胞中的 H2O2 水平来负调节气孔关闭。通过使用 ABA 或 H2O2 处理植物以及脱水，HSL3 的表达显着上调。在功能缺失的 hsl3 突变体中，气孔关闭和阴离子电流激活都对 ABA 处理高度敏感，并且该突变体比野生型更能耐受水分缺乏； HSL3的过度表达则产生相反的效果。在 hsl3 突变体中，参与 H2O2 产生的 NADPH 氧化酶基因 RbohF 的转录显着上调，并且过氧化氢酶活性水平受 ABA 微弱诱导，从而使 H2O2 在保卫细胞中积聚。结论 HSL3 通过保卫细胞中 H2O2 积累引发的 ABA 诱导的气孔关闭来参与对水分缺乏反应的调节。
Guard cells shrink in response to drought stress and abscisic acid (ABA) signaling, thereby reducing stomatal aperture. Hydrogen peroxide (H2O2) is an important signaling molecule acting to induce stomatal closure. As yet, the molecular basis of control over the level of H2O2 in the guard cells remains largely unknown. Here, the leucine-rich repeat (LRR)—receptor-like kinase (RLK) protein HSL3 has been shown to have the ability to negatively regulate stomatal closure by modulating the level of H2O2 in the guard cells. HSL3 was markedly up-regulated by treating plants with either ABA or H2O2, as well as by dehydration. In the loss-of-function hsl3 mutant, both stomatal closure and the activation of anion currents proved to be hypersensitive to ABA treatment, and the mutant was more tolerant than the wild type to moisture deficit; the overexpression of HSL3 had the opposite effect. In the hsl3 mutant, the transcription of NADPH oxidase gene RbohF involved in H2O2 production showed marked up-regulation, as well as the level of catalase activity was weakly inducible by ABA, allowing H2O2 to accumulate in the guard cells. HSL3 was concluded to participate in the regulation of the response to moisture deficit through ABA-induced stomatal closure triggered by the accumulation of H2O2 in the guard cells.
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影响因子：
7.2
作者：
Hunt, L;Mills, LN;Gray, JE
通讯作者：
Gray, JE
DOI：
10.1073/pnas.0907205106
发表时间：
2009-12-01
影响因子：
11.1
作者：
Jammes, Fabien;Song, Charlotte;Kwak, June M.
通讯作者：
Kwak, June M.
DOI：
10.1073/pnas.0912021106
发表时间：
2009-12-15
影响因子：
11.1
作者：
Geiger, Dietmar;Scherzer, Soenke;Hedrich, Rainer
通讯作者：
Hedrich, Rainer
影响因子：
7.4
作者：
Hong, SW;Jon, JH;Nam, HG
通讯作者：
Nam, HG
影响因子：
7.2
作者：
Bright, J;Desikan, R;Neill, SJ
通讯作者：
Neill, SJ