Septins regulate the equatorial dynamics of the separation initiation network kinase Sid2p and glucan synthases to ensure proper cytokinesis
Septins regulate the equatorial dynamics of the separation initiation network kinase Sid2p and glucan synthases to ensure proper cytokinesis
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Septin 调节分离起始网络激酶 Sid2p 和葡聚糖合酶的赤道动力学，以确保正确的胞质分裂。
DOI：
10.1111/febs.14487
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发表时间：
2018-07
期刊：
影响因子：
5.4
通讯作者：
Fu Chuanhai
中科院分区：
文献类型：
--
作者：
Zheng Shengnan;Dong Fenfen;Rasul Faiz;Yao Xuebiao;Jin Quan-wen;Zheng Fan;Fu Chuanhai
间隔蛋白通常起支架和皮质屏障的作用，限制膜蛋白的扩散。在裂殖型裂殖酵母中，在收缩肌球蛋白环(CAR)收缩过程中纺锤体断裂后，间隔蛋白在隔膜形成环状结构，并作为支架招募葡聚糖酶介导最终的子代细胞分离。尽管如此，目前尚不清楚在胞质分裂过程中，在细胞分离之前，间隔蛋白是否发挥了任何重要作用。利用活细胞显微镜，我们仔细研究了SIN(间隔启动网络)信号和葡聚糖合成酶，这是确保CAR正常功能的两个关键因素。在没有核心隔膜成分Spn1p的情况下，紧凑型轿车的形成提前，轿车的收缩速度略有下降，但明显下降。此外，SIN激酶Sid2p和葡聚糖合成酶Bgs1p和Ags1p很早就形成了赤道环，但它们在赤道区的维持减少了SPN1Δ细胞。这些发现表明，Septins通过协调Sid2p和葡聚糖合成酶的赤道动力学，在CAR的准确建立和维护中发挥关键作用。因此，这项工作表明，除了在最终的细胞分离过程中发挥作用外，Septins还在调节早期的细胞动力学事件中发挥重要作用，包括CAR组装和收缩、SIN信号以及葡聚糖合成酶的皮质动力学。
Septins generally function as scaffolds and as cortical barriers to restrict the diffusion of membrane proteins. In the fission yeast Schizosaccharomyces pombe, septins form a ring structure at the septum after spindle breakdown during the constriction of the contractile actomyosin ring (CAR) and serve as a scaffold to recruit glucanases to mediate ultimate daughter cell separation. Despite this, it remains unclear if septins play any significant roles before the cell separation during cytokinesis. Employing live cell microscopy, we carefully examined SIN (Septation Initiation Network) signaling and glucan synthases, two key factors ensuring proper function of the CAR. In the absence of the core septin component Spn1p, the formation of a compact CAR is advanced and the CAR constriction rate is slightly but significantly decreased. Moreover, the SIN kinase Sid2p and the glucan synthases Bgs1p and Ags1p form an equatorial ring quite prematurely, but their maintenance at the equatorial region is diminished spn1Δ cells. These findings suggest that septins act as key players in an accurate establishment and the maintenance of CAR by orchestrating the equatorial dynamics of Sid2p and glucan synthases. Hence, this work demonstrates that, in addition to their function during ultimate cell septation, septins have important roles in regulating earlier cytokinetic events, including CAR assembly and constriction, SIN signaling, and the cortical dynamics of the glucan synthases.
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DOI：
10.1074/jbc.m114.548792
发表时间：
2014-06
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
Sofía Muñoz;E. Manjón;Y. Sánchez
通讯作者：
Sofía Muñoz;E. Manjón;Y. Sánchez
DOI：
10.1083/jcb.201002055
发表时间：
2010-09-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ray S;Kume K;Gupta S;Ge W;Balasubramanian M;Hirata D;McCollum D
通讯作者：
McCollum D
影响因子：
5.4
作者：
Willet AH;McDonald NA;Gould KL
通讯作者：
Gould KL
影响因子：
3.3
作者：
Mulvihill, DP;Barretto, C;Hyams, JS
通讯作者：
Hyams, JS
影响因子：
11.8
作者：
Wu, JQ;Kuhn, JR;Pollard, TD
通讯作者：
Pollard, TD