Diarrhea in young children from low-income countries leads to large-scale alterations in intestinal microbiota composition.
Diarrhea in young children from low-income countries leads to large-scale alterations in intestinal microbiota composition.
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DOI：
10.1186/gb-2014-15-6-r76
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发表时间：
2014-06-27
期刊：
影响因子：
12.3
通讯作者：
Stine OC
中科院分区：
文献类型：
--
作者：
Pop M;Walker AW;Paulson J;Lindsay B;Antonio M;Hossain MA;Oundo J;Tamboura B;Mai V;Astrovskaya I;Corrada Bravo H;Rance R;Stares M;Levine MM;Panchalingam S;Kotloff K;Ikumapayi UN;Ebruke C;Adeyemi M;Ahmed D;Ahmed F;Alam MT;Amin R;Siddiqui S;Ochieng JB;Ouma E;Juma J;Mailu E;Omore R;Morris JG;Breiman RF;Saha D;Parkhill J;Nataro JP;Stine OC
腹泻病仍然是发展中国家婴幼儿发病率和死亡率的主要原因。迫切需要更好地了解新型的、可能未培养的肠道病原体对严重肠道疾病的贡献，以及可能促进严重疾病的正常肠道微生物群组成的扭曲。我们使用高通量16 S rRNA基因测序来比较被诊断患有中度至重度腹泻（MSD）的5岁以下儿童的粪便微生物群组成与无腹泻对照组的微生物群。我们的研究包括来自西非、东非和东南亚四个低收入国家的992名儿童。已知的病原体以及目前不被认为是重要的致脑炎病原体的细菌与MSD呈正相关，这些病原体包括埃希氏菌/志贺氏菌、颗粒状菌属和缓症链球菌/肺炎链球菌群。在这两种情况下和控制，往往有明显的负相关性之间的兼性厌氧谱系和专性厌氧谱系。总体属级微生物群组成显示出对照组中从低水平到高水平的普雷沃氏菌的变化，而在MSD病例中，年幼儿童与年长儿童的大肠杆菌/志贺氏菌从高水平到低水平的变化;然而，许多属之间存在显著的部位和年龄差异。我们的研究结果扩展了目前对发展中国家幼儿微生物群相关腹泻致病性的理解。我们的研究结果必须建立在相关分析的基础上，必须通过流行病学和分子技术进一步验证。
Diarrheal diseases continue to contribute significantly to morbidity and mortality in infants and young children in developing countries. There is an urgent need to better understand the contributions of novel, potentially uncultured, diarrheal pathogens to severe diarrheal disease, as well as distortions in normal gut microbiota composition that might facilitate severe disease. We use high throughput 16S rRNA gene sequencing to compare fecal microbiota composition in children under five years of age who have been diagnosed with moderate to severe diarrhea (MSD) with the microbiota from diarrhea-free controls. Our study includes 992 children from four low-income countries in West and East Africa, and Southeast Asia. Known pathogens, as well as bacteria currently not considered as important diarrhea-causing pathogens, are positively associated with MSD, and these include Escherichia/Shigella, and Granulicatella species, and Streptococcus mitis/pneumoniae groups. In both cases and controls, there tend to be distinct negative correlations between facultative anaerobic lineages and obligate anaerobic lineages. Overall genus-level microbiota composition exhibit a shift in controls from low to high levels of Prevotella and in MSD cases from high to low levels of Escherichia/Shigella in younger versus older children; however, there was significant variation among many genera by both site and age. Our findings expand the current understanding of microbiota-associated diarrhea pathogenicity in young children from developing countries. Our findings are necessarily based on correlative analyses and must be further validated through epidemiological and molecular techniques.
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影响因子：
12.3
作者：
Huse SM;Huber JA;Morrison HG;Sogin ML;Welch DM
通讯作者：
Welch DM
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
168.9
作者：
Kotloff, Karen L.;Nataro, James P.;Levine, Myron M.
通讯作者：
Levine, Myron M.
DOI：
10.1093/cid/cis761
发表时间：
2012-12
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Levine MM;Kotloff KL;Nataro JP;Muhsen K
通讯作者：
Muhsen K
影响因子：
4.2
作者：
Jin D;Chen C;Li L;Lu S;Li Z;Zhou Z;Jing H;Xu Y;Du P;Wang H;Xiong Y;Zheng H;Bai X;Sun H;Wang L;Ye C;Gottschalk M;Xu J
通讯作者：
Xu J