Circulating immune cell and microRNA in patients with uveal melanoma developing metastatic disease.
Circulating immune cell and microRNA in patients with uveal melanoma developing metastatic disease.
复制标题
DOI：
10.1016/j.molimm.2013.11.018
复制
发表时间：
2014-04
影响因子：
3.6
通讯作者：
Triozzi PL
中科院分区：
文献类型：
--
作者：
Achberger S;Aldrich W;Tubbs R;Crabb JW;Singh AD;Triozzi PL
免疫应答与葡萄膜黑色素瘤进展的控制有关。由特定microRNA（miRs）介导的表观遗传机制调节免疫应答。从6名葡萄膜黑色素瘤患者的诊断中抽取血液，当时没有转移的临床或放射学证据，直到转移出现。通过流式细胞术评估循环T细胞、自然杀伤（NK）、自然杀伤T（NKT）和髓系抑制细胞群体。使用免疫磁珠分离CD 3+、CD 15+和CD 56+细胞。通过定量聚合酶链反应测定免疫调节miR的血浆和细胞水平。转移的发生与循环中CD 3 − CD 56 dim NK细胞和CD 8+和双阴性CD 3 + CD 56 + NKT细胞的减少有关。ICOS+ CD 4 + FoxP 3 + T调节细胞和CD 11b + CD 14 − CD 15+髓系抑制细胞增加。与对照组相比，诊断时研究患者中miR-20 a、125 b、146 a、155、181 a和223的血浆水平更高。当出现转移时，miR-20 a、125 b、146 a、155和223的血浆水平升高，而miR-181 a降低。在CD 3+、CD 15+和CD 56+细胞群中也观察到免疫调节miR的改变。葡萄膜黑色素瘤转移的发展与免疫效应细胞和调节细胞的变化有关，这与肿瘤免疫监视的减弱一致。这些变化与免疫调节miR的血浆和细胞水平的变化相关。这些结果可能有助于指导葡萄膜黑色素瘤的免疫治疗和生物标志物的开发。
The immune response has been implicated in the control of uveal melanoma progression. Epigenetic mechanisms mediated by specific microRNAs (miRs) regulate immune responses. Blood was drawn from six patients with uveal melanoma followed from diagnosis, at which time there was no clinical or radiographic evidence of metastasis, until metastasis manifested. Circulating T cell, natural killer (NK), natural killer T (NKT), and myeloid suppressor cell populations were assessed by flow cytometry. CD3+, CD15+, and CD56+ cells were isolated using immunomagnetic beads. Plasma and cellular levels of immune regulatory miRs were determined by quantitative polymerase chain reaction assays. The development of metastasis was associated with decreases in circulating CD3−CD56dim NK cells and CD8+ and double-negative CD3+CD56+ NKT cells. ICOS+CD4+FoxP3+ T regulatory cells and CD11b+CD14−CD15+ myeloid suppressor cells increased. Plasma levels of miR-20a, 125b, 146a, 155, 181a, and 223 were higher in the study patients at diagnosis compared to controls. Plasma levels of miR-20a, 125b, 146a, 155, and 223 increased, and miR-181a decreased when metastasis manifested. Alterations in immune regulatory miRs were also observed in CD3+, CD15+, and CD56+ cell populations. The development of metastasis in uveal melanoma is associated with changes in immune effector and regulatory cells consistent with lessening tumor immune surveillance. These changes are associated with changes in plasma and cellular levels of immune regulatory miRs. The results may help guide uveal melanoma immunotherapy and biomarker development.
登录
查看更多内容
影响因子：
17.8
作者：
Niederkorn, Jerry Y.
通讯作者：
Niederkorn, Jerry Y.
使用miRNA Mimetics在体内重新编程与肿瘤相关的树突状细胞触发了针对卵巢癌的保护性免疫。
影响因子：
11.2
作者：
Cubillos-Ruiz JR;Baird JR;Tesone AJ;Rutkowski MR;Scarlett UK;Camposeco-Jacobs AL;Anadon-Arnillas J;Harwood NM;Korc M;Fiering SN;Sempere LF;Conejo-Garcia JR
通讯作者：
Conejo-Garcia JR
影响因子：
--
作者：
Dithmar, S;Rusciano, D;Grossniklaus, HE
通讯作者：
Grossniklaus, HE
影响因子：
2.2
作者：
Cetrulo Lorenzi, Julio Cesar;Brum, Doralina G.;Silva, Wilson Araujo, Jr.
通讯作者：
Silva, Wilson Araujo, Jr.
影响因子：
15.9
作者：
Eyles, Jo;Puaux, Anne-Laure;Abastado, Jean-Pierre
通讯作者：
Abastado, Jean-Pierre