Role of UCP2 expression after hepatic warm ischemia-reperfusion in the rat.
Role of UCP2 expression after hepatic warm ischemia-reperfusion in the rat.
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DOI：
10.5009/gnl.2011.5.4.486
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发表时间：
2011-12
期刊：
影响因子：
3.4
通讯作者：
Maehara Y
中科院分区：
文献类型：
--
作者：
Ninomiya M;Shirabe K;Shimada M;Terashi T;Maehara Y
解偶联蛋白-2 （UCP2）在肝脏中的作用目前尚不清楚。新出现的证据表明UCP2与氧化应激之间存在关系。在本研究中，我们验证了肝脏中UCP2表达可能在热缺血-再灌注（I/R）过程中根据氧化应激发生变化的假设。Wistar大鼠局部脑缺血40分钟（短缺血）或90分钟（长缺血），再灌注4小时。采用逆转录聚合酶链反应和免疫组织化学方法评估UCP2在缺血和非缺血脑叶中的表达。还比较了肝组织中丙二醛的浓度。长时间缺血组脑缺血叶中丙二醛浓度明显升高。在短缺血组缺血叶中，诱导治疗前未表达UCP2蛋白的肝细胞表达UCP2蛋白，且表达水平高于长缺血组。UCP2在小叶内的分布似乎与坏死区域的分布呈负相关。UCP2的表达，甚至在非缺血的叶中也有类似的小叶内异质性。温I/R后，在肝细胞中诱导UCP2。虽然UCP2表达的原始作用在本质上可能具有细胞保护作用，但其在肝脏I/R中的实际保护作用可能很小
The role of uncoupling protein-2 (UCP2) in the liver is currently unclear. Emerging evidence suggests a relationship between UCP2 and oxidative stress. In the present study, we tested the hypothesis that UCP2 expression in the liver might change during warm ischemia-reperfusion (I/R) according to oxidative stress. Wistar rats were subjected to 40 (short ischemia) or 90 (long ischemia) minutes of partial lobar ischemia followed by 4 hours of reperfusion. UCP2 expression in the ischemic and nonischemic lobes was assessed using reverse transcription-polymerase chain reaction and immunohistochemistry. Malondialdehyde concentrations in the liver tissue were also compared. Malondialdehyde concentrations in the ischemic lobes were significantly higher in the long ischemia group. In the ischemic lobes of the short ischemia group, UCP2 protein expression was induced in hepatocytes, which did not express the protein prior to treatment, and the expression levels were higher than in the long ischemia group. The intralobular distribution of UCP2 seemed to correlate inversely with that of the necrotic area. UCP2 expression was observed, even in nonischemic lobes with similar intralobular heterogeneity. UCP2 was induced in hepatocytes after warm I/R. Although the primitive role of UCP2 expression may be cytoprotective in nature, its actual protective effect in hepatic I/R may be minimal
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影响因子：
30.8
作者：
Fleury, C;Neverova, M;Warden, CH
通讯作者：
Warden, CH
影响因子：
64.8
作者：
Enerback, S;Jacobsson, A;Kozak, LP
通讯作者：
Kozak, LP
影响因子：
2.2
作者：
Kato, Y;Tanaka, J;Koyama, K
通讯作者：
Koyama, K
DOI：
10.1006/bbrc.1997.6852
发表时间：
1997-06-27
影响因子：
3.1
作者：
Larrouy, D;Laharrague, P;Ricquier, D
通讯作者：
Ricquier, D
影响因子：
9.6
作者：
Ninomiya, M;Shimada, M;Maehara, Y
通讯作者：
Maehara, Y