Genome-wide kinetics of nucleosome turnover determined by metabolic labeling of histones.
Genome-wide kinetics of nucleosome turnover determined by metabolic labeling of histones.
复制标题
DOI：
10.1126/science.1186777
复制
发表时间：
2010-05-28
期刊：
影响因子：
--
通讯作者：
Henikoff S
中科院分区：
文献类型：
--
作者：
Deal RB;Henikoff JG;Henikoff S
核小体破坏和替换是维持表观基因组的关键活动，但这些高度动态的过程一直难以研究。在这里，我们描述了一个直接的方法来测量核小体动力学全基因组。我们发现，核小体营业额是最快的活性基因体，表观遗传调控元件，复制起点在果蝇细胞。核小体在三胸组蛋白结合位点的翻转速度比Polycomb组蛋白结合位点的翻转速度更快，这表明核小体的翻转差异是其相反活动的基础，并对依赖于组蛋白标记稳定性的表观遗传模型提出了挑战。我们的研究结果建立了一个通用的策略，研究核小体动力学和发现核小体营业额的差异，在整个基因组可能有功能意义的表观基因组的维护，基因调控和DNA复制的控制。
Nucleosome disruption and replacement are crucial activities that maintain epigenomes, but these highly dynamic processes have been difficult to study. Here, we describe a direct method for measuring nucleosome dynamics genome-wide. We found that nucleosome turnover is most rapid over active gene bodies, epigenetic regulatory elements, and replication origins in Drosophila cells. Nucleosomes turn over faster at sites for trithorax-group than Polycomb-group protein binding, suggesting that nucleosome turnover differences underlie their opposing activities and challenging models for epigenetic inheritance that rely on stability of histone marks. Our results establish a general strategy for studying nucleosome dynamics and uncover nucleosome turnover differences across the genome that are likely to have functional significance for epigenome maintenance, gene regulation, and control of DNA replication.
登录
查看更多内容
影响因子：
4.5
作者：
Nègre N;Brown CD;Shah PK;Kheradpour P;Morrison CA;Henikoff JG;Feng X;Ahmad K;Russell S;White RA;Stein L;Henikoff S;Kellis M;White KP
通讯作者：
White KP
影响因子：
56.9
作者：
Mito, Yoshiko;Henikoff, Jorja G.;Henikoff, Steven
通讯作者：
Henikoff, Steven
影响因子：
16
作者：
Rufiange, Anne;Jacques, Pierre-Etienne;Nourani, Amine
通讯作者：
Nourani, Amine
影响因子：
7
作者：
MacAlpine, Heather K.;Gordan, Raluca;MacAlpine, David M.
通讯作者：
MacAlpine, David M.
影响因子：
16
作者：
Jamai, Adil;Imoberdorf, Rachel Maria;Strubin, Michel
通讯作者：
Strubin, Michel