Spatial Localization and Quantitation of Androgens in Mouse Testis by Mass Spectrometry Imaging.
Spatial Localization and Quantitation of Androgens in Mouse Testis by Mass Spectrometry Imaging.
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DOI：
10.1021/acs.analchem.6b02242
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发表时间：
2016-11-01
影响因子：
7.4
通讯作者：
Andrew, Ruth
中科院分区：
文献类型：
--
作者：
Cobice, Diego F.;Livingstone, Dawn E. W.;Mackay, C. Logan;Goodwin, Richard J. A.;Smith, Lee B.;Walker, Brian R.;Andrew, Ruth
雄激素对男性发育和生殖功能至关重要。它们作为血液传播的内分泌激素被运送到其作用部位，但也可以在组织内产生，以内分泌和旁分泌方式起作用。因此，循环浓度可能无法准确反映特定组织微环境中的雄激素影响。质谱成像允许直接从组织表面对小分子种类进行区域分析。然而，由于电离差和局部离子抑制，类固醇激素难以检测。在此，使用吉拉德T试剂进行衍生化，以电荷标记睾酮和5α-二氢睾酮，从而直接检测小鼠睾丸（基础和最大刺激状态）和大鼠前列腺中的这些类固醇。睾酮的检测限为≤ 0.1 pg。内源性类固醇的示例性检测通过基质辅助激光解吸电离和傅里叶变换离子回旋共振检测（在150 μ m空间分辨率下）或四极杆飞行时间检测（在50 μm空间分辨率下）来实现。通过液体萃取表面分析和电喷雾电离后的碰撞诱导碎片化实现结构确认。该应用拓宽了组织表面衍生化策略的范围，以阐明健康和疾病中的局部内分泌信号。
Androgens are essential for male development and reproductive function. They are transported to their site of action as blood-borne endocrine hormones but can also be produced within tissues to act in intracrine and paracrine fashions. Because of this, circulating concentrations may not accurately reflect the androgenic influence within specific tissue microenvironments. Mass spectrometry imaging permits regional analysis of small molecular species directly from tissue surfaces. However, due to poor ionization and localized ion suppression, steroid hormones are difficult to detect. Here, derivatization with Girard T reagent was used to charge-tag testosterone and 5α-dihydrotestosterone allowing direct detection of these steroids in mouse testes, in both basal and maximally stimulated states, and in rat prostate. Limits of detection were ∼0.1 pg for testosterone. Exemplary detection of endogenous steroids was achieved by matrix-assisted laser desorption ionization and either Fourier transform ion cyclotron resonance detection (at 150 μm spatial resolution) or quadrupole-time-of-flight detection (at 50 μm spatial resolution). Structural confirmation was achieved by collision induced fragmentation following liquid extraction surface analysis and electrospray ionization. This application broadens the scope for derivatization strategies on tissue surfaces to elucidate local endocrine signaling in health and disease.
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影响因子：
4.8
作者：
Rebourcet D;Wu J;Cruickshanks L;Smith SE;Milne L;Fernando A;Wallace RJ;Gray CD;Hadoke PW;Mitchell RT;O'Shaughnessy PJ;Smith LB
通讯作者：
Smith LB
胎儿间质细胞的发育、功能和命运
影响因子：
7.3
作者：
Wen, Qing;Cheng, C. Yan;Liu, Yi-Xun
通讯作者：
Liu, Yi-Xun
影响因子：
4.8
作者：
Mahendroo, MS;Cala, KM;Russell, DW
通讯作者：
Russell, DW
影响因子：
6.1
作者：
Upreti, Rita;Naredo, Gregorio;Faqehi, Abdullah M. M.;Hughes, Katherine A.;Stewart, Laurence H.;Walker, Brian R.;Homer, Natalie Z. M.;Andrew, Ruth
通讯作者：
Andrew, Ruth
影响因子：
2.7
作者：
Shackleton, CHL;Chuang, H;Segura, J
通讯作者：
Segura, J