A fluorescent aptasensing strategy for adenosine triphosphate detection using tris(bipyridine)ruthenium(II) complex containing six cyclodextrin units
A fluorescent aptasensing strategy for adenosine triphosphate detection using tris(bipyridine)ruthenium(II) complex containing six cyclodextrin units
复制标题
使用含有六个环糊精单元的三(联吡啶)钌(II)复合物检测三磷酸腺苷的荧光适体传感策略
DOI：
10.1016/j.cclet.2016.11.013
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发表时间：
2017-03
影响因子：
9.1
通讯作者：
He Pin-Gang
中科院分区：
文献类型：
--
作者：
Nie Xin;Ning Xin;Zhao Ying-Ying;Yang Li-Zhu;Zhang Fan;He Pin-Gang
使用金属环糊精、含有六个环糊精单元的三(联吡啶)钌(II)复合物(6CD-Ru)开发了一种用于检测三磷酸腺苷(ATP)的灵敏无标记荧光适体传感策略，与母体化合物Ru(bpy)3Cl2相比，其表现出更强的发射信号。此外，发射光谱表明，ATP-适体（ssDNA）可以显着增加6CD-Ru的荧光强度，这归因于适体和环糊精之间的相互作用，这可以保护钌核心免受溶液中氧气的发射猝灭。随着ATP的加入，6CD-Ru上适配体与环糊精之间的相互作用减弱，优先形成ATP/适配体复合物，导致荧光强度相应降低，可用于定量检测ATP。在 1 nmol/L 至 1 μmol/L 范围内，荧光与 ATP 浓度对数呈线性关系，检测限为 0.5 nmol/L（S/N = 3）。所提出的荧光适体传感策略表现出高灵敏度和特异性，无需任何标记或扩增程序，并且还可以应用于许多其他适体特异性靶标的检测。
A sensitive label-free fluorescent aptasensing strategy for the detection of adenosine triphosphate (ATP) has been developed with a metallocyclodextrin, tris(bipyridine)ruthenium(II) complex containing six cyclodextrin units (6CD-Ru), which exhibited much stronger emission signal compared to the parent compound Ru(bpy)3Cl2. Furthermore, the emission spectrum showed that the ATP-aptamer (ssDNA) could increase the fluorescence intensity of 6CD-Ru dramatically, attributed to the interaction between aptamer and cyclodextrin, which could provide protection to the ruthenium core from the quenching of emission by oxygen in the solution. With the addition of ATP, the interaction between aptamer and cyclodextrins on 6CD-Ru was diminished, since the ATP/aptamer complex had the priority to be formed, leading to the corresponding reduction of fluorescence intensity, which could be utilized to detect ATP quantitatively. A linear relationship was displayed between the fluorescence and the logarithm of ATP concentrations in the range from 1 nmol/L to 1 μmol/L with the detection limit of 0.5 nmol/L (S/N = 3). The proposed fluorescent aptasensing strategy exhibited high sensitivity and specificity, without any labeling or amplification procedures, and it could also be applied for the detection of many other aptamer-specific targets.
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影响因子：
2.9
作者：
Liu, Fang;Zhang, Juan;Deng, Le
通讯作者：
Deng, Le
基于 SYBR Green I 染料的通用、无标记适体传感器，用于 ATP 和凝血酶的荧光检测
影响因子：
12.6
作者：
Kong, Ling;Xu, Jin;Chai, Yaqin
通讯作者：
Chai, Yaqin
影响因子：
4.9
作者：
Fan Zhang;Yingying Zhao;Hong Chen;Xiuhua Wang;Qiong Chen;P. He
通讯作者：
Fan Zhang;Yingying Zhao;Hong Chen;Xiuhua Wang;Qiong Chen;P. He
影响因子：
12.6
作者：
Kai Zhang;Ke Wang;Minhao Xie;Lan Xu;Xue Zhu;S. Pan;Qun Zhang;Biao Huang
通讯作者：
Kai Zhang;Ke Wang;Minhao Xie;Lan Xu;Xue Zhu;S. Pan;Qun Zhang;Biao Huang
DOI：
10.1039/b806908e
发表时间：
2008-10
期刊：
The Analyst
影响因子：
--
作者：
Yanyan Wang;B. Liu
通讯作者：
Yanyan Wang;B. Liu