Protective Efficacy of Rhomboid-Like Protein 3 as a Candidate Antigen Against Eimeria maxima in Chickens.
Protective Efficacy of Rhomboid-Like Protein 3 as a Candidate Antigen Against Eimeria maxima in Chickens.
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DOI：
10.3389/fmicb.2021.614229
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发表时间：
2021
影响因子：
5.2
通讯作者：
Song X
中科院分区：
文献类型：
--
作者：
Chen C;Tian D;Su J;Liu X;Shah MAA;Li X;Xu L;Yan R;Song X
禽球虫病给全世界养禽业带来巨大的经济损失。第三代疫苗，包括亚单位疫苗和DNA疫苗，展现出良好的发展前景。在之前的一项研究中，我们发现极大艾美耳球虫的菱形样蛋白3（EmROM3）与艾美耳球虫物种的感染有关。然而，EmROM3 对极大艾美耳球虫 (E. maxima) 的保护功效仍不清楚。在本研究中，用重组蛋白 EmROM3 (rEmROM3) 或 pVAX1-EmROM3 对鸡进行肌内免疫，以确定 EmROM3 诱导的免疫反应。通过测量免疫鸡的血清 IgG 抗体水平来确定诱导的体液免疫反应。通过测量免疫相关细胞因子的转录水平和免疫鸡T细胞亚群的比例来检测诱导的细胞免疫反应。最后，通过免疫激发试验评估了 EmROM3 疫苗对巨型艾美球虫的保护功效。结果表明，纯化的rEmROM3与鸡抗大肠杆菌反应。最大血清。 pVAX1-EmROM3重组质粒在接种疫苗的鸡注射的肌肉中进行转录和翻译。与对照组相比，实验组的IgG滴度、CD4+和CD8+T细胞比例以及脾脏细胞因子转录水平均显着升高。免疫挑战试验显示，用 rEmROM3 或 pVAX1-EmROM3 免疫可恢复体重增加、减轻肠道损伤、减少卵囊产量以及较高的抗球虫指数 (ACI)，表明对大球虫有部分保护作用。这些结果表明，EmROM3 是开发针对巨型艾美球菌感染的新型疫苗的有效候选抗原。
Avian coccidiosis brings tremendous economic loss to the poultry industry worldwide. The third generation vaccine, including subunit and DNA vaccines, exhibited promising developmental prospects. In a previous study, we found rhomboid-like protein 3 of Eimeria maxima (EmROM3) was involved in infections by Eimeria species. However, the protective efficacy of EmROM3 against Eimeria maxima (E. maxima) remains unknown. In this study, chickens were intramuscularly immunized with the recombinant protein EmROM3 (rEmROM3) or pVAX1-EmROM3 to determine the EmROM3-induced immune response. The induced humoral immune response was determined by measuring serum IgG antibody levels in immunized chickens. The induced cellular immune response was detected by measuring the transcription level of immune related cytokines and the proportion of T cell subsets of the immunized chickens. Finally, the protective efficacy of the EmROM3 vaccine against E. maxima was evaluated by immunization-challenge trials. Results revealed that the purified rEmROM3 reacted with chicken anti-E. maxima serum. The recombinant plasmid of pVAX1-EmROM3 was transcribed and translated in the injected muscle from the vaccinated chickens. In experimental groups, the IgG titers, proportions of CD4+ and CD8+ T cells, and transcription level of splenic cytokines were significantly increased compared with the control groups. The immunization-challenge trial revealed that immunization with rEmROM3 or pVAX1-EmROM3 led to restored weight gain, alleviated enteric lesion, decreased oocyst output as well as the higher anticoccidial index (ACI), indicating partial protection against E. maxima. These results indicate that EmROM3 is an effective candidate antigen for developing novel vaccines against infection by E. maxima.
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影响因子：
4.9
作者：
Hwang SY;Kim JY;Kim KW;Park MK;Moon Y;Kim WU;Kim HY
通讯作者：
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影响因子：
6.7
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10.1016/j.ijpddr.2014.10.002
发表时间：
2014-12
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