Human brain microvascular endothelial cells resist elongation due to shear stress.
Human brain microvascular endothelial cells resist elongation due to shear stress.
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DOI：
10.1016/j.mvr.2015.02.008
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发表时间：
2015-05
影响因子：
3.1
通讯作者：
Searson, Peter C.
中科院分区：
文献类型：
--
作者：
Reinitz, Adam;DeStefano, Jackson;Ye, Mao;Wong, Andrew D.;Searson, Peter C.
Endothelial cells in straight sections of vessels are known to elongate and align in the direction of flow. This phenotype has been replicated in confluent monolayers of bovine aortic endothelial cells and human umbilical cord endothelial vein cells (HUVECs) in cell culture under physiological shear stress. Here we report on the morphological response of human brain microvascular endothelial cells (HBMECs) in confluent monolayers in response to shear stress. Using a microfluidic platform we image confluent monolayers of HBMECs and HUVECs under shear stresses up to 16 dyne cm−2. From live-cell imaging we quantitatively analyze the cell morphology and cell speed as a function of time. We show that HBMECs do not undergo a classical transition from cobblestone to spindle-like morphology in response to shear stress. We further show that under shear stress, actin fibers are randomly oriented in the cells indicating that there is no cytoskeletal remodeling. These results suggest that HBMECs are programmed to resist elongation and alignment under shear stress, a phenotype that may be associated with the unique properties of the blood–brain barrier.
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影响因子：
4.6
作者：
CHAUDHURI, S;NGUYEN, H;FRANK, CB
通讯作者：
FRANK, CB
DOI：
10.1115/1.3138276
发表时间：
1981-01-01
影响因子：
1.7
作者：
DEWEY, CF;BUSSOLARI, SR;DAVIES, PF
通讯作者：
DAVIES, PF
影响因子：
3.5
作者：
KAMIYA, A;BUKHARI, R;TOGAWA, T
通讯作者：
TOGAWA, T
影响因子：
5.6
作者：
GIRARD, PR;NEREM, RM
通讯作者：
NEREM, RM
DOI：
10.1115/1.2834303
发表时间：
1998-02-01
影响因子：
1.7
作者：
Chiu, JJ;Wang, DL;Usami, S
通讯作者：
Usami, S