Transmural ultrasound imaging of thermal lesion and action potential changes in perfused canine cardiac wedge preparations by high intensity focused ultrasound ablation.
Transmural ultrasound imaging of thermal lesion and action potential changes in perfused canine cardiac wedge preparations by high intensity focused ultrasound ablation.
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DOI：
10.1371/journal.pone.0082689
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Deng CX
中科院分区：
文献类型：
--
作者：
Wu Z;Gudur MS;Deng CX
术中成像对于指导心律失常消融非常重要。很难获得消融过程中热损伤形成与跨壁平面动作电位（AP）变化的术中相关性。本研究使用冠状动脉灌注的犬心室楔形标本（n = 13）测试了高强度聚焦超声（HIFU）消融术中病变和AP变化的透壁成像参数超声成像。 从epi/endoeps表面起搏制备物，并施加HIFU（3.5 MHz，11 Hz脉冲重复频率，70%占空比，持续时间4 s，3500 W/cm 2），在此期间，使用di-4-ANEPPS对楔的相同透壁表面进行同步光学标测（1 kframes/s）和超声成像（30 MHz）。时空相关AP测量和超声成像允许量化AP振幅（阿帕）的降低、50%复极化时AP持续时间的缩短、AP三角测量、光学AP升高的降低以及HIFU损伤内和周围沿着组织深度方向的传导速度变化。与病变相关的阿帕不可逆变化阈值确定为43±1%，受试者工作特征（ROC）曲线下面积（AUC）为0.96±0.01（n = 13）。 对背向散射积分、Rayleigh（α）和对数正态（σ）参数、σ累积极值等超声成像参数进行了检验，其中σ累积极值在检测病变（ROC AUC 0.89±0.01，n = 13）和阿帕变化（ROC AUC 0.79±0.03，n = 13）方面表现最好。 总之，使用光学标测和超声成像识别了HIFU消融中的特征性组织和AP变化，并将其时空相关。使用σ的累积极值的参数超声成像可以检测HIFU损伤和阿帕降低。
Intra-procedural imaging is important for guiding cardiac arrhythmia ablation. It is difficult to obtain intra-procedural correlation of thermal lesion formation with action potential (AP) changes in the transmural plane during ablation. This study tested parametric ultrasound imaging for transmural imaging of lesion and AP changes in high intensity focused ultrasound (HIFU) ablation using coronary perfused canine ventricular wedge preparations (n = 13). The preparations were paced from epi/endocardial surfaces and subjected to HIFU application (3.5 MHz, 11 Hz pulse-repetition-frequency, 70% duty cycle, duration 4 s, 3500 W/cm2), during which simultaneous optical mapping (1 kframes/s) using di-4-ANEPPS and ultrasound imaging (30 MHz) of the same transmural surface of the wedge were performed. Spatiotemporally correlated AP measurements and ultrasound imaging allowed quantification of the reduction of AP amplitude (APA), shortening of AP duration at 50% repolarization, AP triangulation, decrease of optical AP rise, and change of conduction velocity along tissue depth direction within and surrounding HIFU lesions. The threshold of irreversible change in APA correlating to lesions was determined to be 43±1% with a receiver operating characteristic (ROC) area under curve (AUC) of 0.96±0.01 (n = 13). Ultrasound imaging parameters such as integrated backscatter, Rayleigh (α) and log-normal (σ) parameters, cumulative extrema of σ were tested, with the cumulative extrema of σ performing the best in detecting lesion (ROC AUC 0.89±0.01, n = 13) and change of APA (ROC AUC 0.79±0.03, n = 13). In conclusion, characteristic tissue and AP changes in HIFU ablation were identified and spatiotemporally correlated using optical mapping and ultrasound imaging. Parametric ultrasound imaging using cumulative extrema of σ can detect HIFU lesion and APA reduction.
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影响因子：
20.1
作者：
Glukhov AV;Fedorov VV;Lou Q;Ravikumar VK;Kalish PW;Schuessler RB;Moazami N;Efimov IR
通讯作者：
Efimov IR
影响因子：
2.9
作者：
CLIFFORD, L;FITZGERALD, P;JAMES, D
通讯作者：
JAMES, D
DOI：
10.1111/j.1540-8159.1995.tb02523.x
发表时间：
1995-02-01
影响因子：
1.8
作者：
GE, YZ;SHAO, PZ;KADISH, A
通讯作者：
KADISH, A
DOI：
10.1109/tuffc.2002.1049735
发表时间：
2002-11-01
影响因子：
3.6
作者：
Hao, XH;Bruce, CJ;Greenleaf, JF
通讯作者：
Greenleaf, JF
影响因子：
37.8
作者：
Pérez, FJ;Wood, MA;Schubert, CM
通讯作者：
Schubert, CM