Programmed cell death associated with the formation of schizo-lysigenous aerenchyma in Nelumbo nucifera root.
Programmed cell death associated with the formation of schizo-lysigenous aerenchyma in Nelumbo nucifera root.
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DOI：
10.3389/fpls.2022.968841
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
Nelumbo nucifera（N.摘要珠母贝是重要的水生经济作物，具有较高的食用、药用、观赏和生态修复价值。通气组织的形成;荷叶根系是在长期进化过程中对水环境的一种适应性特征。本研究利用光学显微镜、电子显微镜和分子生物学技术，对烟草通气组织的发育过程和细胞学事件进行了研究。本文研究了在外源21%氧、乙烯（ET）和ET合成抑制剂1-甲基环丙烯（1-MCP）处理下，银莲花（Nucifera）根通气组织形成的动态变化。结果表明，在通气组织形成过程中，N.荷叶根形成期出现质膜内陷和空泡膜破裂，随后出现核变形、染色质浓缩和边缘化，此时末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）检测呈阳性。在通气组织发育的扩展阶段，细胞质降解，细胞质中出现许多小泡，细胞器开始降解。然后质膜开始降解，细胞壁的降解是PCD的最后一步。在根际环境中持续通入21%的氧气后，根际环境中的氮素含量明显降低。nucifera根的通气组织面积;荷叶根比对照组小。ET诱导N.但通气组织面积也比对照增大。相反，1-MCP在一定程度上抑制了通气组织的发生。因此，N. nucifera根是PCD的产物，其形成方式为次生溶生。低氧环境能诱导植物通气组织的形成。ET信号参与了N.并具有正调控作用。本研究为植物通气组织的形成机制提供了相关数据，也为探索植物通气组织形成的调控机制提供了细胞学基础。
Nelumbo nucifera (N. nucifera) is an important aquatic economic crop with high edible, medicinal, ornamental, and ecological restoration values. Aerenchyma formation in N. nucifera root is an adaptive trait to the aquatic environment in long-term evolution. In this study, light microscopy, electron microscopy, and molecular biology techniques were used to study the process of the aerenchyma development and cytological events in N. nucifera root and the dynamic changes of aerenchyma formation under the treatment of exogenous 21% oxygen, ethylene (ET), and ET synthesis i + nhibitor 1-methylcyclopropene (1-MCP). The results showed that programmed cell death (PCD) occurred during the aerenchyma formation in N. nucifera root. Plasmalemma invagination and vacuole membrane rupture appeared in the formation stage, followed by nuclear deformation, chromatin condensation and marginalization, and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) detection was positive at this time. In the expansion stage of the aerenchyma development, cytoplasmic degradation and many vesicles appeared in the cytoplasm, and organelles began to degrade. Then the plasma membrane began to degrade, and the degradation of the cell wall was the last PCD step. After 21% oxygen was continuously filled in the rhizosphere environment of N. nucifera roots, the area of aerenchyma in N. nucifera roots was smaller than that in the control group. Moreover, ET induced the earlier occurrence of aerenchyma in N. nucifera root, but also, the area of aerenchyma became larger than that of the control. On the contrary, 1-MCP inhibited the occurrence of aerenchyma to some extent. Therefore, the formation of aerenchyma in N. nucifera root resulted from PCD, and its formation mode was schizo-lysigenous. A hypoxic environment could induce aerenchyma formation in plants. ET signal was involved in aerenchyma formation in N. nucifera root and had a positive regulatory effect. This study provides relevant data on the formation mechanism of plant aerenchyma and the cytological basis for exploring the regulation mechanism of plant aerenchyma formation.
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影响因子：
11.6
作者：
Gunawardena, AHLAN;Greenwood, JS;Dengler, NG
通讯作者：
Dengler, NG
影响因子：
4.3
作者：
Gunawardena, AHLAN;Pearce, DM;Evans, DE
通讯作者：
Evans, DE
影响因子：
4.2
作者：
Dolferus, R;Ellis, M;Peacock, WJ
通讯作者：
Peacock, WJ
DOI：
10.3390/molecules23112856
发表时间：
2018-11-02
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Zhao Z;Li Y;Zhao S;Zhang J;Zhang H;Fu B;He F;Zhao M;Liu P
通讯作者：
Liu P
影响因子：
2.8
作者：
Abiko, Tomomi;Miyasaka, Susan C.
通讯作者：
Miyasaka, Susan C.