Integrative Omics Analysis of Three Oil Palm Varieties Reveals (Tanzania × Ekona) TE as a Cold-Resistant Variety in Response to Low-Temperature Stress.
Integrative Omics Analysis of Three Oil Palm Varieties Reveals (Tanzania × Ekona) TE as a Cold-Resistant Variety in Response to Low-Temperature Stress.
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三个油棕品种的综合组学分析揭示（坦桑尼亚和 Ekona）TE 作为应对低温胁迫的抗寒品种
DOI：
10.3390/ijms232314926
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发表时间：
2022-11-29
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
油棕（Elaeis guineensis Jacq.）是一种经济上重要的热带油料作物，在世界各地的热带地区广泛种植。作为一种热带作物，低温胁迫对油棕产生不利影响。然而，综合叶转录组学和蛋白质组学分析尚未进行在寒冷胁迫下的油棕榈作物。在这项研究中，整合组学转录组学和基于iTRAQ的蛋白质组学方法被用于三个油棕品种，即，B × E（巴门达× Ekona）、O × G（E. oleifera × Elaeisguineensis）和T × E（Tanzania × Ekona）对低温胁迫的响应。响应于（8 °C）下5天的低温胁迫，在BE-0_VS_BE-5中鉴定出总共5175个上调和2941个下调的DEG，在OG-0_VS_OG-5中鉴定出总共3468个上调和2443个下调的DEG，在TE-0_VS_TE-5中鉴定出总共3667个上调和2151个下调的DEG。基于iTRAQ的蛋白质组学分析显示，与在28 °C和8 °C下处理的对照样品相比，BE-0_VS_BE-5有349个上调和657个下调的DEP，OG-0_VS_OG-5有372个上调和264个下调的DEP，TE-0_VS_TE-5有500个上调和321个下调的DEP。油棕的KEGG途径相关性研究表明，油棕的代谢合成和次生代谢产物的生物合成途径在转录组和蛋白质组中显著富集。相关性表达模式显示，TE-0_VS_TE-5在转录组和蛋白质组中高度表达，而BE-0_VS_BE-5在低温下受到抑制。此外，许多转录因子（TF）被发现可能在低温下调节三个油棕品种的冷驯化。此外，这项研究确定了蛋白质参与压力（非生物，生物，氧化和热休克），光合作用和呼吸的iTRAQ为基础的蛋白质组学分析的三个油棕品种。胁迫响应蛋白丰度的增加和光合相关蛋白丰度的减少表明，TE品种可能成为抗寒响应低温胁迫。本研究为进一步了解我国油棕品种适应低温胁迫的分子机制奠定了基础。
Oil palm (Elaeis guineensis Jacq.) is an economically important tropical oil crop widely cultivated in tropical zones worldwide. Being a tropical crop, low-temperature stress adversely affects the oil palm. However, integrative leaf transcriptomic and proteomic analyses have not yet been conducted on an oil palm crop under cold stress. In this study, integrative omics transcriptomic and iTRAQ-based proteomic approaches were employed for three oil palm varieties, i.e., B × E (Bamenda × Ekona), O × G (E. oleifera × Elaeis guineensis), and T × E (Tanzania × Ekona), in response to low-temperature stress. In response to low-temperature stress at (8 °C) for 5 days, a total of 5175 up- and 2941 downregulated DEGs in BE-0_VS_BE-5, and a total of 3468 up- and 2443 downregulated DEGs for OG-0_VS_OG-5, and 3667 up- and 2151 downregulated DEGs for TE-0_VS_TE-5 were identified. iTRAQ-based proteomic analysis showed 349 up- and 657 downregulated DEPs for BE-0_VS_BE-5, 372 up- and 264 downregulated DEPs for OG-0_VS_OG-5, and 500 up- and 321 downregulated DEPs for TE-0_VS_TE-5 compared to control samples treated at 28 °C and 8 °C, respectively. The KEGG pathway correlation of oil palm has shown that the metabolic synthesis and biosynthesis of secondary metabolites pathways were significantly enriched in the transcriptome and proteome of the oil palm varieties. The correlation expression pattern revealed that TE-0_VS_TE-5 is highly expressed and BE-0_VS_BE-5 is suppressed in both the transcriptome and proteome in response to low temperature. Furthermore, numerous transcription factors (TFs) were found that may regulate cold acclimation in three oil palm varieties at low temperatures. Moreover, this study identified proteins involved in stresses (abiotic, biotic, oxidative, and heat shock), photosynthesis, and respiration in iTRAQ-based proteomic analysis of three oil palm varieties. The increased abundance of stress-responsive proteins and decreased abundance of photosynthesis-related proteins suggest that the TE variety may become cold-resistant in response to low-temperature stress. This study may provide a basis for understanding the molecular mechanism for the adaptation of oil palm varieties in response to low-temperature stress in China.
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