Proteomic analyses reveal differences in cold acclimation mechanisms in freezing-tolerant and freezing-sensitive cultivars of alfalfa.
Proteomic analyses reveal differences in cold acclimation mechanisms in freezing-tolerant and freezing-sensitive cultivars of alfalfa.
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DOI：
10.3389/fpls.2015.00105
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发表时间：
2015
影响因子：
5.6
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Chen J;Han G;Shang C;Li J;Zhang H;Liu F;Wang J;Liu H;Zhang Y
紫花苜蓿（Medicago sativa L.）在抵御严冬中起着至关重要的作用。为了研究耐寒苜蓿（ZD）和抗冻敏感苜蓿（W5）的冷驯化机制，从叶片中提取全蛋白和低丰度蛋白（去除RuBisCO后），分析蛋白质水平上的差异。共筛选出84个样点，鉴定出67个样点。其中，ZD和W5中分别有49个和24个斑点的丰度在适应低温胁迫过程中发生了变化。蛋白质组学研究结果表明，参与光合作用、蛋白质代谢、能量代谢、胁迫和氧化还原等过程的蛋白质在低温胁迫下发生了动员。在ZD中，在蛋白质中观察到更多的变化，并且响应于低温胁迫，自体代谢和生物合成减慢，从而减少消耗，允许体内平衡。W5的蛋白质折叠和蛋白质合成能力增强，这使得其能够抵御低温胁迫。耐冻苜蓿对低温的感知能力比冻敏苜蓿更敏感。该蛋白质组学研究为紫花苜蓿冷驯化机制提供了新的视角。
Cold acclimation in alfalfa (Medicago sativa L.) plays a crucial role in cold tolerance to harsh winters. To examine the cold acclimation mechanisms in freezing-tolerant alfalfa (ZD) and freezing-sensitive alfalfa (W5), holoproteins, and low-abundance proteins (after the removal of RuBisCO) from leaves were extracted to analyze differences at the protein level. A total of 84 spots were selected, and 67 spots were identified. Of these, the abundance of 49 spots and 24 spots in ZD and W5, respectively, were altered during adaptation to chilling stress. Proteomic results revealed that proteins involved in photosynthesis, protein metabolism, energy metabolism, stress and redox and other proteins were mobilized in adaptation to chilling stress. In ZD, a greater number of changes were observed in proteins, and autologous metabolism and biosynthesis were slowed in response to chilling stress, thereby reducing consumption, allowing for homeostasis. The capability for protein folding and protein biosynthesis in W5 was enhanced, which allows protection against chilling stress. The ability to perceive low temperatures was more sensitive in freezing-tolerant alfalfa compared to freezing-sensitive alfalfa. This proteomics study provides new insights into the cold acclimation mechanism in alfalfa.
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影响因子：
3.4
作者：
Giavalisco, P;Nordhoff, E;Gobom, J
通讯作者：
Gobom, J
影响因子：
3.5
作者：
ARRIGONI, O;DIPIERRO, S;BORRACCINO, G
通讯作者：
BORRACCINO, G
影响因子：
3.3
作者：
Grimaud, Florent;Renaut, Jenny;Goulas, Estelle
通讯作者：
Goulas, Estelle
影响因子：
4.3
作者：
Bocian, Aleksandra;Kosmala, Arkadiusz;Zwierzykowski, Zbigniew
通讯作者：
Zwierzykowski, Zbigniew
影响因子：
5.6
作者：
Budiman C;Koga Y;Takano K;Kanaya S
通讯作者：
Kanaya S