Age-related neurochemical changes in the rhesus macaque superior olivary complex.
Age-related neurochemical changes in the rhesus macaque superior olivary complex.
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DOI：
10.1002/cne.23427
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发表时间：
2013-12-20
影响因子：
2.5
通讯作者：
Recanzone, Gregg H.
中科院分区：
文献类型：
--
作者：
Gray, Daniel T.;Engle, James R.;Recanzone, Gregg H.
阳性免疫反应的钙结合蛋白小清蛋白（PV）和一氧化氮合酶NADPH-黄递酶（NADPH d）是有据可查的啮齿类和灵长类动物的中枢听觉系统的神经元内。这些蛋白质被认为在听觉处理的调节中发挥作用。研究这些蛋白质表达的年龄相关变化主要在啮齿动物中进行，但在灵长类动物模型中很少。在脑干中，上级橄榄复合体（SOC）对于方位角中的声源定位的计算是至关重要的，并且与年龄相关的听力缺陷的一个标志是定位声音的能力降低。为了研究这些组织化学标记物如何随着年龄和听力损失而变化，我们研究了8只年龄从12岁到35岁的恒河猴。在麻醉动物中获得了对短声和纯音刺激的听觉脑干反应（ABR）。然后对这些相同动物的脑干进行PV和NADPHd反应性染色。计数SOC三个核团中的反应性神经元，并计算每种细胞类型的密度。我们发现PV和NADPHd表达在内侧上级橄榄体中随着年龄和ABR阈值的增加而增加，但在斜方体内侧核或外侧上级橄榄体中则没有。总之，这些结果表明，SOC所采用的蛋白质表达的变化可能会补偿老年灵长类动物听觉灵敏度的功效损失。
Positive immunoreactivity to the calcium-binding protein parvalbumin (PV) and nitric oxide synthase NADPH-diaphorase (NADPHd) is well documented within neurons of the central auditory system of both rodents and primates. These proteins are thought to play roles in the regulation of auditory processing. Studies examining the age-related changes in expression of these proteins have been conducted primarily in rodents but are sparse in primate models. In the brainstem, the superior olivary complex (SOC) is crucial for the computation of sound source localization in azimuth, and one hallmark of age-related hearing deficits is a reduced ability to localize sounds. To investigate how these histochemical markers change as a function of age and hearing loss, we studied eight rhesus macaques ranging in age from 12 to 35 years. Auditory brainstem responses (ABRs) were obtained in anesthetized animals for click and tone stimuli. The brainstems of these same animals were then stained for PV and NADPHd reactivity. Reactive neurons in the three nuclei of the SOC were counted, and the densities of each cell type were calculated. We found that PV and NADPHd expression increased with both age and ABR thresholds in the medial superior olive but not in either the medial nucleus of the trapezoid body or the lateral superior olive. Together these results suggest that the changes in protein expression employed by the SOC may compensate for the loss of efficacy of auditory sensitivity in the aged primate.
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影响因子：
3.9
作者：
CASPARY, DM;MILBRANDT, JC;HELFERT, RH
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作者：
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