Self-Driven Jamming in Growing Microbial Populations.
Self-Driven Jamming in Growing Microbial Populations.
复制标题
DOI：
10.1038/nphys3741
复制
发表时间：
2016-08
期刊：
影响因子：
19.6
通讯作者：
中科院分区：
文献类型：
--
作者：
在自然环境中，微生物倾向于在密集的种群中生长，在那里它们需要推动周围环境，以容纳新细胞的空间。相关的接触力在各种种群水平的过程中发挥着关键作用，包括生物膜的形成、多孔介质的定植和生物组织的入侵。尽管机械力的特征是单细胞水平的，但如何由单细胞力量的组合产生集体推动力仍然令人费解。在这里，我们揭示了一种集体的限制机制，我们称之为自驱动干扰，它促进了微生物种群中巨大机械压力的积累。在空间有限的环境中对萌芽酵母种群进行的微流控实验表明，自驱动干扰是由于微生物增殖驱动的力链的逐渐形成和突然崩溃，扩展了驱动颗粒物的框架。由此产生的接触压力可能会变得足够大，从而减缓细胞的生长，推迟细胞在G1期的周期，并通过裂纹扩展使微环境紧张甚至破坏。我们的结果表明，自驱动堵塞和大机械压力的积累是微生物在受限空间中生长的自然趋势，有助于微生物的发病和生物污染。
In natural settings, microbes tend to grow in dense populations where they need to push against their surroundings to accommodate space for new cells. The associated contact forces play a critical role in a variety of population-level processes, including biofilm formation, the colonization of porous media, and the invasion of biological tissues. Although mechanical forces have been characterized at the single cell level, it remains elusive how collective pushing forces result from the combination of single cell forces. Here, we reveal a collective mechanism of confinement, which we call self-driven jamming, that promotes the build-up of large mechanical pressures in microbial populations. Microfluidic experiments on budding yeast populations in space-limited environments show that self-driven jamming arises from the gradual formation and sudden collapse of force chains driven by microbial proliferation, extending the framework of driven granular matter. The resulting contact pressures can become large enough to slow down cell growth, to delay the cell cycle in the G1 phase, and to strain or even destroy the microenvironment through crack propagation. Our results suggest that self-driven jamming and build-up of large mechanical pressures is a natural tendency of microbes growing in confined spaces, contributing to microbial pathogenesis and biofouling.
登录
查看更多内容
DOI：
10.1073/pnas.1212429109
发表时间：
2012-11-13
影响因子：
11.1
作者：
Asally, Munehiro;Kittisopikul, Mark;Sueel, Guerol M.
通讯作者：
Sueel, Guerol M.
影响因子：
3.4
作者：
Delarue, Morgan;Monte, Fabien;Cappello, Giovanni
通讯作者：
Cappello, Giovanni
生物膜积累对多孔介质流体力学的影响
影响因子：
11.4
作者：
CUNNINGHAM, AB;CHARACKLIS, WG;CRAWFORD, D
通讯作者：
CRAWFORD, D
影响因子：
9.8
作者：
Charvin G;Oikonomou C;Siggia ED;Cross FR
通讯作者：
Cross FR
影响因子：
--
作者：
Basan, Markus;Risler, Thomas;Prost, Jacques
通讯作者：
Prost, Jacques