Isolation and characterization of low-sulphur-tolerant mutants of Arabidopsis.
Isolation and characterization of low-sulphur-tolerant mutants of Arabidopsis.
复制标题
拟南芥耐低硫突变体的分离和鉴定
DOI：
10.1093/jxb/erq161
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发表时间：
2010-07
影响因子：
6.9
通讯作者：
Xiang CB
中科院分区：
文献类型：
--
作者：
Wu Y;Zhao Q;Gao L;Yu XM;Fang P;Oliver DJ;Xiang CB
硫是植物生长和发育以及抵御生物和非生物胁迫的必需元素。提高硫酸盐利用效率是作物改良的一个重要问题。关于硫酸盐利用效率的遗传决定因素知之甚少。迄今为止，尚未报道具有改善的SUE的功能获得性突变体。在这里，两个低硫耐受突变体，sue 3和sue 4的分离和表征报告使用高通量遗传筛选，其中设计了一个“无硫”的固体培养基，以提供必要的选择压力，以抑制野生型幼苗的生长。这两个突变体表现出改善的耐受性低硫条件和发达的根系。sue 3和sue 4的突变体表型都是硫酸盐缺乏所特有的，突变体表现出对重金属和氧化胁迫的耐受性增强。遗传分析表明，sue 3是由一个隐性核突变引起的，而sue 4是由一个显性核突变引起的。sue 3中的隐性基因座是先前鉴定的VirE 2相互作用蛋白1。sue 4的优势位点是T-DNA上的4个增强子激活的功能未知位点。SUE 3和SUE 4在低硫耐性中的功能通过多突变等位基因或重演分析得到证实。综上所述，我们的研究结果表明，这种遗传筛选是一种合理的方法来分离拟南芥突变体，具有改善的低硫耐受性和潜在的提高硫酸盐利用效率。在sue 3和sue 4中鉴定的两个位点有助于理解耐低硫的分子机制。
Sulphur is an essential element for plant growth and development as well as for defence against biotic and abiotic stresses. Increasing sulphate utilization efficiency (SUE) is an important issue for crop improvement. Little is known about the genetic determinants of sulphate utilization efficiency. No gain-of-function mutants with improved SUE have been reported to date. Here the isolation and characterization of two low-sulphur-tolerant mutants, sue3 and sue4 are reported using a high-throughput genetic screen where a ‘sulphur-free’ solid medium was devised to give the selection pressure necessary to suppress the growth of the wild-type seedlings. Both mutants showed improved tolerance to low sulphur conditions and well-developed root systems. The mutant phenotype of both sue3 and sue4 was specific to sulphate deficiency and the mutants displayed enhanced tolerance to heavy metal and oxidative stress. Genetic analysis revealed that sue3 was caused by a single recessive nuclear mutation while sue4 was caused by a single dominant nuclear mutation. The recessive locus in sue3 is the previously identified VirE2-interacting Protein 1. The dominant locus in sue4 is a function-unknown locus activated by the four enhancers on the T-DNA. The function of SUE3 and SUE4 in low sulphur tolerance was confirmed either by multiple mutant alleles or by recapitulation analysis. Taken together, our results demonstrate that this genetic screen is a reasonable approach to isolate Arabidopsis mutants with improved low sulphur tolerance and potentially with enhanced sulphate utilization efficiency. The two loci identified in sue3 and sue4 should assist in understanding the molecular mechanisms of low sulphur tolerance.
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影响因子：
11.6
作者：
Hamburger, D;Rezzonico, E;Poirier, Y
通讯作者：
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影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
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7.2
作者：
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通讯作者：
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影响因子：
7.4
作者：
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通讯作者：
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影响因子：
7.4
作者：
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通讯作者：
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