Paper-based electrochemiluminescent screening for genotoxic activity in the environment.
Paper-based electrochemiluminescent screening for genotoxic activity in the environment.
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DOI：
10.1021/es304426j
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发表时间：
2013-02-19
影响因子：
11.4
通讯作者：
Rusling, James F.
中科院分区：
文献类型：
--
作者：
Mani, Vigneshwaran;Kadimisetty, Karteek;Malla, Spundana;Joshi, Amit A.;Rusling, James F.
采用丝网印刷电极和蜡纸热转印的方法制作了一种低成本的微流控纸电化学器件（μPED）。μPED具有发光钌金属聚合物、微粒体代谢酶和DNA的薄膜，可检测环境样品中潜在的遗传毒性污染物活性。与检测特定污染化合物的传统分析方法不同，μPED旨在快速测量环境样品中存在的遗传毒性等效物，并将与苯并[a]芘（B[a]P）相关的信号作为参考标准。分析终点是使用电荷耦合器件（CCD）相机测量的电化学发光输出检测器械中产生的代谢物的DNA损伤。该测量的概念验证是针对烟、水和食品样品建立的。μPED为现场环境监测提供了一种快速筛查工具，专门监测样品中有毒化合物代谢物的遗传毒性反应性。
A low cost, microfluidic paper electrochemical device (μPED) was fabricated using screen printing of electrodes and heat transfer of patterned wax paper onto filter paper. The μPED features films of a light-emitting ruthenium metallopolymer, microsomal metabolic enzymes, and DNA to detect potential genotoxic pollutant activity in environmental samples. Unlike conventional analytical methods that detect specific pollutant compounds, the μPED was designed to rapidly measure the presence of genotoxic equivalents in environmental samples with the signal related to benzo[a]pyrene (B[a]P) as a reference standard. The analytical endpoint is the detection of DNA damage from metabolites produced in the device using an electrochemiluminescence output measured with a charge-coupled device (CCD) camera. Proof-of-concept of this measurement was established for smoke, water and food samples. The μPED provides a rapid screening tool for on-site environmental monitoring that specifically monitors the genotoxic reactivity of metabolites of toxic compounds present in the samples.
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