Transcriptional signatures of influenza A/H1N1-specific IgG memory-like B cell response in older individuals.
Transcriptional signatures of influenza A/H1N1-specific IgG memory-like B cell response in older individuals.
复制标题
老年人甲型/H1N1 流感特异性 IgG 记忆样 B 细胞反应的转录特征。
DOI：
10.1016/j.vaccine.2016.06.034
复制
发表时间：
2016-07-25
期刊：
影响因子：
5.5
通讯作者：
Poland GA
中科院分区：
文献类型：
--
作者：
Haralambieva IH;Ovsyannikova IG;Kennedy RB;Zimmermann MT;Grill DE;Oberg AL;Poland GA
研究表明，对甲型H1N1流感病毒的基于回忆的体液免疫应答起源于激活的记忆B细胞。本研究的目的是鉴定与流感疫苗接种后记忆B细胞应答相关的基线、早期和晚期血液转录特征（在外周血单核细胞/PBMC中）。我们对159名受试者（50-74岁）接种含有A/加州/7/2009/H1N1样病毒的季节性三价流感疫苗后的样本进行了接种前和接种后mRNA-Seq转录谱分析，并进行了惩罚回归建模，以确定与接种后流感A/H1N1特异性记忆B细胞ELISPOT反应的相关性。基因组和基因（p值范围为7.92E-08至0.00018，q值范围为0.00019至0.039），证明存在显著相关性（基因表达水平）与记忆B细胞反应的关系表明代谢的重要性（胆固醇和脂质代谢相关），细胞迁移/粘附，MAP激酶，流感疫苗接种后记忆B细胞应答发展中的NF-κ B细胞信号传导（趋化因子/细胞因子信号传导）和转录调控基因特征。通过无偏倚的全转录组分析方法，我们的研究确定了流感疫苗接种后记忆B细胞应答的特征，突出了代谢变化（在其他免疫功能相关事件中）在流感疫苗诱导的免疫记忆调节中未被充分认识的作用。
Studies suggest that the recall-based humoral immune responses to influenza A/H1N1 originates from activated memory B cells. The aim of this study was to identify baseline, early and late blood transcriptional signatures (in peripheral blood mononuclear cells/PBMCs) associated with memory B cell response following influenza vaccination. We used pre- and post-vaccination mRNA-Seq transcriptional profiling on samples from 159 subjects (50–74 years old) following receipt of seasonal trivalent influenza vaccine containing the A/California/7/2009/H1N1-like virus, and penalized regression modeling to identify associations with influenza A/H1N1-specific memory B cell ELISPOT response after vaccination. Genesets and genes (p-value range 7.92E-08 to 0.00018, q-value range 0.00019 to 0.039) demonstrating significant associations (of gene expression levels) with memory B cell response suggest the importance of metabolic (cholesterol and lipid metabolism-related), cell migration/adhesion, MAP kinase, NF-kB cell signaling (chemokine/cytokine signaling) and transcriptional regulation gene signatures in the development of memory B cell response after influenza vaccination. Through an unbiased transcriptome-wide profiling approach, our study identified signatures of memory B cell response following influenza vaccination, highlighting the underappreciated role of metabolic changes (among the other immune function-related events) in the regulation of influenza vaccine-induced immune memory.
登录
查看更多内容
影响因子：
13.1
作者：
McElhaney, Janet E.
通讯作者：
McElhaney, Janet E.
影响因子：
9.1
作者：
Agrawal, Sudhanshu;Gupta, Sudhir
通讯作者：
Gupta, Sudhir
影响因子：
7
作者：
Oberg AL;Kennedy RB;Li P;Ovsyannikova IG;Poland GA
通讯作者：
Poland GA
DOI：
10.1126/science.1179050
发表时间：
2009-10-09
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Amit I;Garber M;Chevrier N;Leite AP;Donner Y;Eisenhaure T;Guttman M;Grenier JK;Li W;Zuk O;Schubert LA;Birditt B;Shay T;Goren A;Zhang X;Smith Z;Deering R;McDonald RC;Cabili M;Bernstein BE;Rinn JL;Meissner A;Root DE;Hacohen N;Regev A
通讯作者：
Regev A
影响因子：
3.7
作者：
Haralambieva IH;Oberg AL;Ovsyannikova IG;Kennedy RB;Grill DE;Middha S;Bot BM;Wang VW;Smith DI;Jacobson RM;Poland GA
通讯作者：
Poland GA