A genetically modified protein-based hydrogel for 3D culture of AD293 cells.
A genetically modified protein-based hydrogel for 3D culture of AD293 cells.
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用于 AD293 细胞 3D 培养的转基因蛋白质水凝胶
DOI：
10.1371/journal.pone.0107949
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhou H
中科院分区：
文献类型：
--
作者：
Du X;Wang J;Diao W;Wang L;Long J;Zhou H
水凝胶具有良好的生物相容性，可作为药物和细胞的支架材料，在药物释放、组织工程和细胞治疗等方面具有广阔的应用前景。在这项研究中，我们通过在TIP 1的一级结构中引入两个或四个半胱氨酸残基，创建了基于遗传修饰的税收相互作用蛋白-1（TIP 1）的混合水凝胶。引入的半胱氨酸残基与其臂端用马来酰亚胺残基封端的四臂聚（乙二醇）（4-臂-PEG-Mal）交联以形成水凝胶。在一种形式的遗传修饰中，我们掺入了肽序列“GRGDSP”以将生物活性引入蛋白质，并且所得水凝胶可以为AD 293细胞的三维细胞培养提供优良的环境。培养3天后，细胞继续分裂，呈多角体或梭形。此外，AD 293细胞可以很容易地从细胞-凝胶构建体中分离出来，用于未来的大规模培养，培养3天后，用胰蛋白酶处理水凝胶。这项工作大大扩展了用于水凝胶形成的重组蛋白的工具箱，我们相信我们的水凝胶将对那些从事细胞治疗和受控药物递送的人产生相当大的兴趣。
Hydrogels have strong application prospects for drug delivery, tissue engineering and cell therapy because of their excellent biocompatibility and abundant availability as scaffolds for drugs and cells. In this study, we created hybrid hydrogels based on a genetically modified tax interactive protein-1 (TIP1) by introducing two or four cysteine residues in the primary structure of TIP1. The introduced cysteine residues were crosslinked with a four-armed poly (ethylene glycol) having their arm ends capped with maleimide residues (4-armed-PEG-Mal) to form hydrogels. In one form of the genetically modification, we incorporated a peptide sequence ‘GRGDSP’ to introduce bioactivity to the protein, and the resultant hydrogel could provide an excellent environment for a three dimensional cell culture of AD293 cells. The AD293 cells continued to divide and displayed a polyhedron or spindle-shape during the 3-day culture period. Besides, AD293 cells could be easily separated from the cell-gel constructs for future large-scale culture after being cultured for 3 days and treating hydrogel with trypsinase. This work significantly expands the toolbox of recombinant proteins for hydrogel formation, and we believe that our hydrogel will be of considerable interest to those working in cell therapy and controlled drug delivery.
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影响因子：
14
作者：
Branco, Monica C.;Pochan, Darrin J.;Wagner, Norman J.;Schneider, Joel P.
通讯作者：
Schneider, Joel P.
影响因子：
7.4
作者：
Gu, Zhen;Zhao, Muxun;Tang, Yi
通讯作者：
Tang, Yi
影响因子：
14
作者：
Almany, L;Seliktar, D
通讯作者：
Seliktar, D
DOI：
10.1073/pnas.0737381100
发表时间：
2003-04-29
影响因子：
11.1
作者：
Lutolf, MP;Lauer-Fields, JL;Hubbell, JA
通讯作者：
Hubbell, JA
基于短肽的分子水凝胶：新型凝胶化策略及其在组织工程和药物输送中的应用
影响因子：
6.7
作者：
Wang, Huaimin;Yang, Zhimou
通讯作者：
Yang, Zhimou