Enrichment and purification of flavones from rhizomes of Abacopteris penangiana by macroporous resins
Enrichment and purification of flavones from rhizomes of Abacopteris penangiana by macroporous resins
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大孔树脂富集纯化蛇麻子根茎中的黄酮类化合物
DOI：
10.3724/sp.j.1009.2012.00119
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发表时间：
2012-03
影响因子：
4.6
通讯作者：
Yang Chun
中科院分区：
文献类型：
--
作者：
Wei Han;Ruan Jin-Lan;Lei Yong-Fang;Yang Chun
摘要目的研究大孔树脂富集纯化槟榔根茎黄酮的工艺。方法采用静态吸附和解吸实验选择合适的树脂。在选定的HPD500树脂上进行了动力学吸附和解吸实验，优化了黄酮类化合物的分离工艺。通过L4/3正交实验，考察了吸附流速、洗脱液流速、洗脱水体积和乙醇体积4个参数对吸附效果的影响。最后，比较了HPD500处理前后样品的ABTS（2,2′-氮杂比-3-乙基苯并噻唑啉-6-磺酸）自由基清除能力。结果结果表明：初始浓度2.86 mgmL−1，洗脱液为70%乙醇，吸收流速为1 mLmin−1，洗脱液流速为2 mLmin−1，洗脱水为5 BV，洗脱乙醇为5 BV。结论经HPD500处理后，黄酮类化合物的含量可达60%以上，表明大孔树脂可成功用于黄酮类化合物的富集纯化。
Abstract Aim To investigate the enrichment and purification of flavones from the rhizomes of Abacopteris penangiana (RAP) by macroporous resins. Methods Static adsorption and desorption tests were performed to select the appropriate resin. The kinetic adsorption and desorption experiments were carried out on selected HPD500 resin to optimize the separation process of flavones. Additionally, the effects of four parameters including adsorption flow rate, elute flow rate, volume of water and ethanol solution for elution were explored by a L4/3 orthogonal experiment. Finally, the ABTS (2, 2′-azinobis-3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging activities of samples before and after being treated by HPD500 were compared. Results The results showed that the optimal parameters were initial concentration of 2.86 mgmL −1 , elute solution of 70% ethanol, absorb flow rate of 1 mLmin −1 , elute flow rate of 2 mLmin −1 , 5 BV of water for elution and 5 BV of ethanol solution for elution. Conclusion The content of flavones is above 60% in RAP after being treated by HPD500, indicating that macroporous resins could be successfully applied to enrich and purify flavones in RAP.
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影响因子：
5.1
作者：
Zhongxiang Zhao;J. Ruan;Jing Jin;Jian-hua Zou;Dao-nian Zhou;Wei Fang;Fa-Quan Zeng
通讯作者：
Zhongxiang Zhao;J. Ruan;Jing Jin;Jian-hua Zou;Dao-nian Zhou;Wei Fang;Fa-Quan Zeng
DOI：
10.1016/j.jchromb.2008.04.016
发表时间：
2008-05-15
影响因子：
3
作者：
Zhang, Bin;Yang, Ruiyuan;Liu, Chun-Zhao
通讯作者：
Liu, Chun-Zhao
影响因子：
3.4
作者：
Zhongxiang Zhao;J. Ruan;Jing Jin;Chenchen Zhu;Ying-xiang Liu
通讯作者：
Zhongxiang Zhao;J. Ruan;Jing Jin;Chenchen Zhu;Ying-xiang Liu
影响因子：
3.8
作者：
Ying Zhang;Shu-fen Li;Xiwen Wu;Xingang Zhao
通讯作者：
Ying Zhang;Shu-fen Li;Xiwen Wu;Xingang Zhao
影响因子：
1.7
作者：
Zhongxiang Zhao;J. Ruan;Jing Jin;Chen-chen Zhu;Chaozhan Lin
通讯作者：
Zhongxiang Zhao;J. Ruan;Jing Jin;Chen-chen Zhu;Chaozhan Lin