Iterative design of peptide-based hydrogels and the effect of network electrostatics on primary chondrocyte behavior.
Iterative design of peptide-based hydrogels and the effect of network electrostatics on primary chondrocyte behavior.
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DOI：
10.1016/j.biomaterials.2012.06.097
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发表时间：
2012-10
期刊：
影响因子：
14
通讯作者：
Schneider, Joel P.
中科院分区：
文献类型：
--
作者：
Sinthuvanich, Chomdao;Haines-Butterick, Lisa A.;Nagy, Katelyn J.;Schneider, Joel P.
采用迭代肽设计生成两种基于肽的水凝胶，研究网络静电对原代软骨细胞行为的影响。MAX8和HLT2肽的形式电荷态分别为+7和+5。这些肽经过触发折叠和自组装，形成具有相似流变行为和局部网络形态的水凝胶网络，但具有不同的静电特性。每种凝胶可用于直接包封和注射器输送细胞。评估了网络静电对包封和递送后细胞活力、细胞外基质沉积、基因表达以及凝胶细胞结构的整体力学性能随培养时间的变化的影响。电正性较低的HLT2凝胶提供了一个更有利于软骨细胞包封、递送和表型维持的微环境。这种凝胶的细胞活力更高，虽然有中等数量的细胞去分化为成纤维细胞样表型，但许多细胞保留了它们的软骨细胞行为。因此，用HLT2制备的凝胶细胞构建体，在体外静态条件下培养，含有更多的GAG和II型胶原，从而产生机械上优越的构建体。在电阳性程度更高的MAX8凝胶中递送的软骨细胞在包封和递送过程中经历了更大程度的细胞死亡，剩余的活细胞不太容易维持其表型。结果，MAX8凝胶细胞构建的细胞较少，其中能够形成软骨特异性ECM的细胞数量有限。
Iterative peptide design was used to generate two peptide-based hydrogels to study the effect of network electrostatics on primary chondrocyte behavior. MAX8 and HLT2 peptides have formal charge states of +7 and +5 per monomer, respectively. These peptides undergo triggered folding and self-assembly to afford hydrogel networks having similar rheological behavior and local network morphologies, yet different electrostatic character. Each gel can be used to directly encapsulate and syringe-deliver cells. The influence of network electrostatics on cell viability after encapsulation and delivery, extracellular matrix deposition, gene expression, and the bulk mechanical properties of the gel-cell constructs as a function of culture time was assessed. The less electropositive HLT2 gel provides a microenvironment more conducive to chondrocyte encapsulation, delivery, and phenotype maintenance. Cell viability was higher for this gel and although a moderate number of cells dedifferentiated to a fibroblast-like phenotype, many retained their chondrocytic behavior. As a result, gel-cell constructs prepared with HLT2, cultured under static in vitro conditions, contained more GAG and type II collagen resulting in mechanically superior constructs. Chondrocytes delivered in the more electropositive MAX8 gel experienced a greater degree of cell death during encapsulation and delivery and the remaining viable cells were less prone to maintain their phenotype. As a result, MAX8 gel-cell constructs had fewer cells, of which a limited number were capable of laying down cartilage-specific ECM.
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影响因子：
14
作者：
Branco, Monica C.;Pochan, Darrin J.;Wagner, Norman J.;Schneider, Joel P.
通讯作者：
Schneider, Joel P.
影响因子：
2.9
作者：
LABARCA, C;PAIGEN, K
通讯作者：
PAIGEN, K
影响因子：
7
作者：
Hoemann, CD;Sun, J;Buschmann, MD
通讯作者：
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影响因子：
4.9
作者：
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通讯作者：
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DOI：
10.1073/pnas.0701980104
发表时间：
2007-05-08
影响因子：
11.1
作者：
Haines-Butterick, Lisa;Rajagopal, Karthikan;Schneider, Joel P.
通讯作者：
Schneider, Joel P.