Over-expression and localization of a host protein on the membrane of Cryptosporidium parvum infected epithelial cells.
Over-expression and localization of a host protein on the membrane of Cryptosporidium parvum infected epithelial cells.
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DOI：
10.1016/j.molbiopara.2009.07.004
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发表时间：
2009-11
影响因子：
1.5
通讯作者：
Widmer, Giovanni
中科院分区：
文献类型：
--
作者：
Yang, Yi-Lin;Serrano, Myrna G.;Sheoran, Abhineet S.;Manque, Patricio A.;Buck, Gregory A.;Widmer, Giovanni
隐孢子虫属包括几种肠道原虫，它们在肠道上皮细胞中繁殖。用DNA微阵列杂交技术研究了这种感染对培养宿主细胞转录组的影响。用RT-PCR验证了在感染细胞单层中持续上调或下调的14个基因的表达。利用免疫荧光技术，我们检测了由上调基因之一编码的蛋白酶激活受体-2的表达。在受感染的细胞中，该受体定位于宿主细胞膜，该膜覆盖细胞内滋养体和分生孢子。这一观察表明，寄主细胞膜的组成受到滋养体发育的影响，这一现象以前没有描述过。
The genus Cryptosporidium includes several species of intestinal protozoan parasites which multiply in intestinal epithelial cells. The impact of this infection on the transcriptome of cultured host cells was investigated using DNA microarray hybridizations. The expression of 14 genes found to be consistently up- or down-regulated in infected cell monolayers was validated with RT PCR. Using immunofluorescence we examined the expression of Protease Activated Receptor-2, which is encoded by one of the up-regulated genes. In infected cells this receptor localized to the host cell membrane which covers intracellular trophozoites and meronts. This observation indicates that the composition of the host cell membrane is affected by the developing trophozoite, a phenomenon which has not been described previously.
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影响因子：
4
作者：
Deng, MQ;Lancto, CA;Abrahamsen, MS
通讯作者：
Abrahamsen, MS
影响因子：
4.8
作者：
Blader, IJ;Manger, ID;Boothroyd, JC
通讯作者：
Boothroyd, JC
DOI：
10.1152/ajpgi.1999.277.3.g599
发表时间：
1999-09-01
影响因子：
4.5
作者：
Chen, XM;Gores, GJ;LaRusso, NF
通讯作者：
LaRusso, NF
影响因子：
14.8
作者：
Huang, Da Wei;Sherman, Brad T.;Lempicki, Richard A.
通讯作者：
Lempicki, Richard A.
影响因子：
3.1
作者：
Feng, Hanping;Nie, Weijia;Tzipori, Saul
通讯作者：
Tzipori, Saul