Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source.
Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source.
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以乙酸为碳源的重组大肠杆菌微生物生产甲羟戊酸
DOI：
10.1080/21655979.2017.1323592
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发表时间：
2018-01-01
期刊：
影响因子：
4.9
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Xu X;Xie M;Zhao Q;Xian M;Liu H
ABSTRACT We sought to produce mevalonate, an important organic acid, by recombinant Escherichia coli, using acetic acid, a less costly alternative feedstock, as a carbon source. In this study, the mevalonate biosynthesis pathway originating with acetate was constructed in recombinant E. coli, resulting in the production of 1.06 g L−1 mevalonate with a productivity of 0.03 g L−1 h−1 in a 5-L bioreactor. The mevalonate concentration and productivity were significantly enhanced with increased cell density during 2-stage aerobic fermentation, reaching 7.85 g L−1 and 0.13 g L−1 h−1, respectively. Fed-batch fermentation was further optimized under anaerobic and microaerobic conditions, and mevalonate concentrations reached 3.05 g L−1 and 4.97 g L−1, respectively, indicating that the oxygen supply exerts a large impact on mevalonate production from acetate. This study describes a method with high potential to produce mevalonate with the engineered E. coli strain XU143 using the less costly alternative feedstock acetate as a carbon source.
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影响因子：
3.7
作者：
Yang J;Xian M;Su S;Zhao G;Nie Q;Jiang X;Zheng Y;Liu W
通讯作者：
Liu W
DOI：
10.1016/0923-0467(95)03065-4
发表时间：
1996-05-01
期刊：
CHEMICAL ENGINEERING JOURNAL AND THE BIOCHEMICAL ENGINEERING JOURNAL
影响因子：
--
作者：
Baheri, HR;Hill, GA;Roesler, WJ
通讯作者：
Roesler, WJ
影响因子：
56.9
作者：
Zhu, GP;Golding, GB;Dean, AM
通讯作者：
Dean, AM
影响因子：
56.9
作者：
Periana, RA;Mironov, O;Jones, CJ
通讯作者：
Jones, CJ
影响因子：
2.7
作者：
Tabata, K;Hashimoto, SI
通讯作者：
Hashimoto, SI