Microcantilevers modified by specific peptide for selective detection of trimethylamine.
Microcantilevers modified by specific peptide for selective detection of trimethylamine.
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DOI:
10.1016/j.bios.2011.09.001
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发表时间:
2011-12-15
影响因子:
12.6
通讯作者:
Zhu, Shuifang
中科院分区:
文献类型:
--
作者:
Huang, Xin;Li, Mingfu;Xu, Xiaohe;Chen, Hongjun;Ji, Hai-Feng;Zhu, Shuifang
We report a biosensor based on a microcantilever that is modified by a specific peptide for highly selective detection of trimethylamine (TMA). The assay is based on binding-induced bending of the peptide functionalized microcantilevers. The sensor is selectively responsive to TMA. The amplitude of microcantilever bending at equilibrium is a function of the concentration of TMA with a dynamic range from 8 ppm to 800 ppm. The detection limit is approximately 8 ppm. There is a good intra-sensor and an acceptable inter-sensor reproducibility as evidenced by the standard deviation of 5% and 15%, respectively.
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