Microcantilevers modified by specific peptide for selective detection of trimethylamine.

Microcantilevers modified by specific peptide for selective detection of trimethylamine.
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DOI:
10.1016/j.bios.2011.09.001
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发表时间:
2011-12-15
影响因子:
12.6
通讯作者:
Zhu, Shuifang
Zhu, Shuifang
中科院分区:
工程技术1区
文献类型:
--
作者:
Huang, Xin;Li, Mingfu;Xu, Xiaohe;Chen, Hongjun;Ji, Hai-Feng;Zhu, Shuifang

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我们报道了一种基于微悬臂的生物传感器,该传感器被一种特定肽修饰,用于高选择性检测三甲胺(TMA)。该分析是基于肽功能化微悬臂的结合诱导弯曲。该传感器对TMA有选择性响应。微悬臂梁在平衡状态下的弯曲幅度是TMA浓度的函数,动态范围从8ppm到800ppm。检测限约为8 ppm。有一个良好的传感器内部和可接受的传感器之间的再现性,分别为5%和15%的标准偏差证明。
We report a biosensor based on a microcantilever that is modified by a specific peptide for highly selective detection of trimethylamine (TMA). The assay is based on binding-induced bending of the peptide functionalized microcantilevers. The sensor is selectively responsive to TMA. The amplitude of microcantilever bending at equilibrium is a function of the concentration of TMA with a dynamic range from 8 ppm to 800 ppm. The detection limit is approximately 8 ppm. There is a good intra-sensor and an acceptable inter-sensor reproducibility as evidenced by the standard deviation of 5% and 15%, respectively.
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