Laminin in cultured mouse C1300 neuroblastoma cells: immunocytochemical localization by pre- and postembedding electron microscope procedures.
Laminin in cultured mouse C1300 neuroblastoma cells: immunocytochemical localization by pre- and postembedding electron microscope procedures.
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培养的小鼠 C1300 神经母细胞瘤细胞中的层粘连蛋白：通过嵌入前和嵌入后电子显微镜程序进行免疫细胞化学定位。
DOI：
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发表时间：
1983
影响因子：
3.2
通讯作者：
P. Liesi
中科院分区：
文献类型：
--
作者：
P. Liesi
应用过氧化物酶-抗过氧化物酶包埋前电镜技术，对培养的小鼠C1300神经母细胞瘤细胞层粘连蛋白进行了定位。结果进行了比较，通过间接免疫荧光法和胶体金第二抗体法上的Epon包埋切片。层粘连蛋白存在于细胞膜、粗面内质网和胞浆内空泡中。神经母细胞瘤细胞的质膜显示片状定位的层粘连蛋白，显然是参与细胞与基板的连接和间隙连接样细胞间的连接。在正常情况下，高尔基池不含层粘连蛋白。然而，用微摩尔浓度的莫能菌素预处理细胞，导致层粘连蛋白在高尔基体池内积累。这些结果支持层粘连蛋白在培养的神经母细胞瘤细胞中作为粘附蛋白的作用，并表明层粘连蛋白通过高尔基复合体转运。
Laminin was localized in cultured mouse C1300 neuroblastoma cells by applying the peroxidase-antiperoxidase technique in preembedding electron microscopy. The results were compared to those obtained by indirect immunofluorescence and by the colloidal gold second antibody method on Epon-embedded ultrathin sections. Laminin was found in the cell membranes and within the rough endoplasmic reticulum as well as in intracytoplasmic vacuoles. Plasma membranes of the neuroblastoma cells showed a patchy localization of laminin that was apparently involved in cell-to-substrate attachment and in gap junction-like intercellular connections. Under normal conditions, the Golgi cisternae contained no laminin. Pretreatment of cells with micromolar concentrations of monensin, however, lead to an accumulation of laminin within the Golgi cisternae. These results support a role for laminin as an adhesion protein in cultured neuroblastoma cells and indicate that laminin is transported through the Golgi complex.
M1536-B3 细胞中巢蛋白和 GP-2 的产生和分布。
影响因子：
3.7
作者：
Bender,BL;Carlin,B;Jaffe,R;Temple,T;Chung,AE
通讯作者：
Chung,AE
DOI：
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发表时间：
1981-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Carlin;R. Jaffe;B. Bender;A. Chung
通讯作者：
B. Carlin;R. Jaffe;B. Bender;A. Chung