Exploitation of Malus EST-SSRs and the utility in evaluation of genetic diversity in Malus and Pyrus
Exploitation of Malus EST-SSRs and the utility in evaluation of genetic diversity in Malus and Pyrus
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苹果 EST-SSR 的开发及其在苹果和梨遗传多样性评估中的应用
DOI：
10.1007/s10722-009-9524-1
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发表时间：
2010-02
影响因子：
2
通讯作者：
Zheng, X.
中科院分区：
文献类型：
--
作者：
Wang, K.;Cao, Y.;Yao, L.;Cai, D.;Gao, Y.;Teng, Y.;Zheng, X.
通过对Malus EST数据库的筛选，共获得8117条适合的ssr序列，其中二肽型ssr序列是最丰富的重复基序，其中以CT/TC序列为主，其次是AG/GA序列。根据合适的引物序列，我们开发了147对SSR引物，其中94对在预期大小范围内扩增，65对经初步测试发现多态性。利用随机选取的18对引物进一步评价了20个苹果种或品种的亲缘关系。结果表明，这些引物具有较高的多态性，平均每个位点有6.94个等位基因，UPGMA聚类分析将20份苹果材料分为5类，相似度为0.6800，与传统分类基本一致。随后，将这94对引物在4份梨属材料上进行测试，评估标记的可转移性，并从72个功能性SSR中筛选出40个产生多态性扩增子，从中随机选择8个SSR位点，分析梨属植物的遗传多样性和亲缘关系。8对引物在苹果中产生的条带大小相近，平均每个位点有7.375个等位基因。不同位点的杂合度范围为0.29 (MES96) ~ 0.83 (MES138)，平均值为0.55，低于梨基因组源SSR标记分析结果的0.63。UPGMA树形图与先前使用RAPD和AFLP标记获得的结果相似。结果表明，这些EST-SSR标记在苹果属和梨属中均表现出可靠的扩增和多态性，为苹果属和遗传封闭属的遗传研究奠定了基础。
A total of 8117 suitable SSR-contaning ESTs were acquired by screening from a Malus EST database, among which dinudeotide SSRs were the most abundant repeat motif, within which, CT/TC followed by AG/GA were predominant. Based on the suitable sequences, we developed 147 SSR primer pairs, of which 94 pairs gave amplifications within the expected size range while 65 pairs were found to be polymorphic after a preliminary test. Eighteen primer pairs selected randomly were further used to assess genetic relationship among 20 Malus species or cultivars. As a result, these primers displayed high level of polymorphism with a mean of 6.94 alleles per locus and UPGMA cluster analysis grouped twenty Malus accessions into five groups at the similarity level of 0.6800 that were largely congruent to the traditional taxonomy. Subsequently, all of the 94 primer pairs were tested on four accessions of Pyrus to evaluate the transferability of the markers, and 40 of 72 functional SSRs produced polymorphic amplicons from which 8 SSR loci selected randomly were employed to analyze genetic diversity and relationship among a collection of Pyrus. The 8 primer pairs produced expected bands with the similar size in apples with an average of 7.375 alleles per locus. The observed heterozygosity of different loci ranged from 0.29 (MES96) to 0.83 (MES138), with a mean of 0.55 which is lower than 0.63 reported in genome-derived SSR marker analysis in Pyrus. The UPGMA dendrogram was similar to the previous results obtained by using RAPD and AFLP markers. Our results showed that these EST-SSR markers displayed reliable amplification and considerable polymorphism in both Malus and Pyrus, and will contribute to the knowledge of genetic study of Malus and genetically closed genera.
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DOI：
--
发表时间：
1990
期刊：
--
影响因子：
--
作者：
I. Kajiura;Y. Sato
通讯作者：
I. Kajiura;Y. Sato
影响因子：
5.4
作者：
Scott, KD;Eggler, P;Henry, RJ
通讯作者：
Henry, RJ
影响因子：
2
作者：
Jia, Xiao-Ping;Shi, Yun-Su;Li, Yu
通讯作者：
Li, Yu
DOI：
10.1016/s0379-4172(06)60060-7
发表时间：
2006-04-01
期刊：
Acta Genetica Sinica
影响因子：
--
作者：
Jiang Dong;Zhong Guang-Yan;Hong Qi-Bing
通讯作者：
Hong Qi-Bing
影响因子：
5.4
作者：
Pierantoni, L;Cho, KH;Sansavini, S
通讯作者：
Sansavini, S