Did you choose appropriate tracer for retrograde tracing of retinal ganglion cells? The differences between cholera toxin subunit B and Fluorogold.
Did you choose appropriate tracer for retrograde tracing of retinal ganglion cells? The differences between cholera toxin subunit B and Fluorogold.
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您是否选择了合适的示踪剂来逆行追踪视网膜神经节细胞？
DOI：
10.1371/journal.pone.0205133
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Xia X
中科院分区：
文献类型：
--
作者：
Yao F;Zhang E;Gao Z;Ji H;Marmouri M;Xia X
霍乱毒素亚单位B（CT B）和荧光金（FG）是两种广泛应用于视网膜神经节细胞（RGC）数量和功能的逆行示踪剂。然而，这些示踪剂的相对优点和缺点仍然不清楚，这可能导致其不适当的应用。本研究通过将示踪剂分别注入大鼠的上级视丘（SC），1周或2周后处死大鼠，收集其视网膜、脑和视神经。第1 ~ 2周，FG在SC中标记的RGCs数量和弥散面积均大于CTB，CTB在SC中标记的RGCs数量和弥散面积均显著增加，但FG之间无显著性差异，CTB在SC中标记的RGCs数量和弥散面积均显著增加，且标记的RGCs突起数量和长度均大于FG，但相同迹线之间无显著性差异; CTB标记的视神经比FG标记的清晰。结论：CTB和FG均可用于1周或2周大鼠RGCs的逆行标记。FG实现逆行标记的RGCs比CTB更多，而CTB更好地描绘RGCs的形态。此外，CTB似乎更适合逆行标记脑中某些RGC亚型投射其轴突的一些小的非成像核团。
Cholera toxin subunit B (CTB) and Fluorogold(FG) are two widely utilized retrograde tracers to assess the number and function of retinal ganglion cells (RGCs). However, the relative advantages and disadvantages of these tracers remain unclear, which may lead to their inappropriate application. In this study, we compared these tracers by separately injecting the tracer into the superior Colliculi (SC) in rats, one or 2 weeks later, the rats were sacrificed, and their retinas, brains, and optic nerves were collected. From the first to second week, FG displayed a greater number of labeled RGCs and a larger diffusion area in the SC than CTB; The number of CTB labeled RGCs and the diffusion area of CTB in the SC increased significantly, but there was no distinction between FG; Furthermore, CTB exhibited more labeled RGC neurites and longer neurites than FG, but no difference was evident between the same trace; The optic nerves labeled using CTB were much clearer than those labeled using FG. In conclusion, both CTB and FG can be used for the retrograde labeling of RGCs in rats at 1 or 2 weeks. FG achieves retrograde labeling of a greater number of RGCs than CTB, whereas CTB better delineates the morphology of RGCs. Furthermore, CTB seems more suitable for retrograde labeling of some small, non-image forming nuclei in the brain to which certain RGC subtypes project their axons.
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