Neurological function following intra-neural injection of fluorescent neuronal tracers in rats.
Neurological function following intra-neural injection of fluorescent neuronal tracers in rats.
复制标题
大鼠神经内注射荧光神经元示踪剂后的神经功能
DOI：
10.3969/j.issn.1673-5374.2013.14.001
复制
发表时间：
2013-05-15
影响因子：
6.1
通讯作者：
Gu J
中科院分区：
文献类型：
--
作者：
Hu W;Liu D;Zhang Y;Shen Z;Gu T;Gu X;Gu J
当用于长期标记时，荧光神经元示踪剂不应对神经系统产生毒性。以前的研究已经解决了示踪剂的毒性，但将示踪剂注射到完整的神经中是否会导致功能损害仍有待阐明。本研究通过加压注射5%荧光金、4%真蓝和10%氟宝石(5μL)对大鼠的胫神经进行了运动、感觉和自主神经功能的检测。采用步行轨迹分析、足底试验和激光多普勒血流成像等方法评价荧光神经元示踪剂的作用。此外，还观察了神经病理和肌肉湿重比。结果显示，注射荧光金后，大鼠运动神经功能明显丧失，足底敏感性降低，血流量增加，神经源性血管扩张增强。荧光金组有髓神经纤维变性，边界不清，逆行标记效率高。True Blue组也表现出明显的神经源性血管扩张，但运动功能丧失和变性程度较轻，标记的运动神经元较少。氟红组未见运动神经和感觉神经功能异常，无有髓神经纤维变性。实验结果表明，荧光金示踪可导致运动、感觉和自主神经的明显功能损害，而真蓝示踪的功能损害不那么严重。氟红宝石注射剂似乎对神经功能没有影响。
Fluorescent neuronal tracers should not be toxic to the nervous system when used in long-term labeling. Previous studies have addressed tracer toxicity, but whether tracers injected into an intact nerve result in functional impairment remains to be elucidated. In the present study, we examined the functions of motor, sensory and autonomic nerves following the application of 5% Fluoro-Gold, 4% True Blue and 10% Fluoro-Ruby (5 μL) to rat tibial nerves via pressure injection. A set of evaluation methods including walking track analysis, plantar test and laser Doppler perfusion imaging was used to determine the action of the fluorescent neuronal tracers. Additionally, nerve pathology and ratio of muscle wet weight were also observed. Results showed that injection of Fluoro-Gold significantly resulted in loss of motor nerve function, lower plantar sensibility, increasing blood flow volume and higher neurogenic vasodilatation. Myelinated nerve fiber degeneration, unclear boundaries in nerve fibers and high retrograde labeling efficacy were observed in the Fluoro-Gold group. The True Blue group also showed obvious neurogenic vasodilatation, but less severe loss of motor function and degeneration, and fewer labeled motor neurons were found compared with the Fluoro-Gold group. No anomalies of motor and sensory nerve function and no myelinated nerve fiber degeneration were observed in the Fluoro-Ruby group. Experimental findings indicate that Fluoro-Gold tracing could lead to significant functional impairment of motor, sensory and autonomic nerves, while functional impairment was less severe following True Blue tracing. Fluoro-Ruby injection appears to have no effect on neurological function.
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影响因子：
4.8
作者：
de Ruiter, Godard C.;Spinner, Robert J.;Windebank, Anthony J.
通讯作者：
Windebank, Anthony J.
影响因子：
2.6
作者：
Lewin, SL;Utley, DS;Terris, DJ
通讯作者：
Terris, DJ
影响因子：
3
作者：
Puigdellívol-Sánchez, A;Valero-Cabré, A;Molander, C
通讯作者：
Molander, C
影响因子：
2.4
作者：
Haninec P;Kaiser R;Bobek V;Dubový P
通讯作者：
Dubový P
DOI：
10.1073/pnas.1110854108
发表时间：
2011-09-13
影响因子：
11.1
作者：
Beier, Kevin T.;Saunders, Arpiar;Cepko, Constance L.
通讯作者：
Cepko, Constance L.