Comparison of longitudinal in vivo measurements of retinal nerve fiber layer thickness and retinal ganglion cell density after optic nerve transection in rat.
Comparison of longitudinal in vivo measurements of retinal nerve fiber layer thickness and retinal ganglion cell density after optic nerve transection in rat.
复制标题
DOI：
10.1371/journal.pone.0113011
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Fortune B
中科院分区：
文献类型：
--
作者：
Choe TE;Abbott CJ;Piper C;Wang L;Fortune B
目的：探讨单侧视神经切断(ONT)后视网膜神经纤维层厚度(RNFLT)与视网膜神经节细胞(RGC)密度的体内纵向测量之间的关系。将19只成年棕色-挪威大鼠分为N = 10 ONT+RGC标记、N = 3 ONT+车辆(SANS标记)、N = 6 Sham ONT+RGC标记。用光谱域光学相干断层扫描(SD-OCT)测量RNFLT，然后每周测量一次，持续1个月。在ONT或SHAM手术前48小时，逆行上丘霍乱毒素B(CTB)标记视网膜神经节细胞。用激光共聚焦扫描眼底镜(CSLO)测量视网膜神经节细胞密度，每周1个月。死后荧光显微镜下观察视网膜小胶质细胞(抗IBA1)和星形胶质细胞(抗GFAP)的RGC密度和反应性。视网膜神经细胞密度在ONT后2、3、4周分别下降17%(P<；0.0001)、30%(P<；0.0001)和36%(P<；0.0001)；RGC密度在ONT后1、2、3和4周分别下降18%、69%、85%和92%(P<；0.0001)。4周活体测量的RGC密度与死后显微镜下的RGC密度有很强的相关性(R = 0.91，p<；0.0001)。活体测量的RNFlt和RGC密度有很强的相关性(R = 0.81，p<；0.0001)。ONT-CTB标记的对侧眼在2、3、4周时RNFLT分别增加了18%、52%和36%(p<；0.0001)，而未标记ONT-CTB的对侧眼RNFlt无变化。ONT-CTB对侧眼RNFL有明显的小胶质细胞增多症，超过其他对侧眼。在体测量RNFlt和RGC密度具有很强的相关性，可以用来监测视神经损伤后的纵向变化。仅在CTB标记存在的ONT对侧眼中观察到的强烈的同眼效应包括与视网膜小胶质细胞增多症相关的RNFLT的显著增加。
To determine the relationship between longitudinal in vivo measurements of retinal nerve fiber layer thickness (RNFLT) and retinal ganglion cell (RGC) density after unilateral optic nerve transection (ONT). Nineteen adult Brown-Norway rats were studied; N = 10 ONT plus RGC label, N = 3 ONT plus vehicle only (sans label), N = 6 sham ONT plus RGC label. RNFLT was measured by spectral domain optical coherence tomography (SD-OCT) at baseline then weekly for 1 month. RGCs were labeled by retrograde transport of fluorescently conjugated cholera toxin B (CTB) from the superior colliculus 48 hours prior to ONT or sham surgery. RGC density measurements were obtained by confocal scanning laser ophthalmoscopy (CSLO) at baseline and weekly for 1 month. RGC density and reactivity of microglia (anti-Iba1) and astrocytes (anti-GFAP) were determined from post mortem fluorescence microscopy of whole-mount retinae. RNFLT decreased after ONT by 17% (p<0.05), 30% (p<0.0001) and 36% (p<0.0001) at weeks 2, 3 and 4. RGC density decreased after ONT by 18%, 69%, 85% and 92% at weeks 1, 2, 3 and 4 (p<0.0001 each). RGC density measured in vivo at week 4 and post mortem by microscopy were strongly correlated (R = 0.91, p<0.0001). In vivo measures of RNFLT and RGC density were strongly correlated (R = 0.81, p<0.0001). In ONT- CTB labeled fellow eyes, RNFLT increased by 18%, 52% and 36% at weeks 2, 3 and 4 (p<0.0001), but did not change in fellow ONT-eyes sans CTB. Microgliosis was evident in the RNFL of the ONT-CTB fellow eyes, exceeding that observed in other fellow eyes. In vivo measurements of RNFLT and RGC density are strongly correlated and can be used to monitor longitudinal changes after optic nerve injury. The strong fellow eye effect observed in eyes contralateral to ONT, only in the presence of CTB label, consisted of a dramatic increase in RNFLT associated with retinal microgliosis.
登录
查看更多内容
影响因子：
4.4
作者：
Geng Y;Greenberg KP;Wolfe R;Gray DC;Hunter JJ;Dubra A;Flannery JG;Williams DR;Porter J
通讯作者：
Porter J
影响因子：
4.4
作者：
Guo, Li;Normando, Eduardo M.;Cordeiro, M. Francesca
通讯作者：
Cordeiro, M. Francesca
影响因子：
4.4
作者：
Gabriele, Michelle L.;Ishikawa, Hiroshi;Wollstein, Gadi
通讯作者：
Wollstein, Gadi
影响因子：
1.4
作者：
Harwerth, Ronald S.
通讯作者：
Harwerth, Ronald S.
影响因子：
4.4
作者：
Higashide, Tomomi;Kawaguchi, Ichiro;Sugiyama, Kazuhisa
通讯作者：
Sugiyama, Kazuhisa