Forms and Lability of Phosphorus in Algae and Aquatic Macrophytes Characterized by Solution (31)P NMR Coupled with Enzymatic Hydrolysis.
Forms and Lability of Phosphorus in Algae and Aquatic Macrophytes Characterized by Solution (31)P NMR Coupled with Enzymatic Hydrolysis.
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溶液 31P NMR 与酶水解相结合表征藻类和水生植物中磷的形态和稳定性
DOI：
10.1038/srep37164
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发表时间：
2016-11-16
影响因子：
4.6
通讯作者：
Giesy JP
中科院分区：
文献类型：
--
作者：
Feng W;Zhu Y;Wu F;He Z;Zhang C;Giesy JP
溶液磷 31 核磁共振 (31P NMR) 光谱与市售磷酸酶的酶水解 (EH) 相结合，用于表征富营养化湖泊中主要水生植物和藻类提取物中的磷 (P) 化合物。水生植物和藻类的总可提取有机磷 (Po) 浓度范围分别为 504 至 1643 mg kg−1 和 2318 至 8395 mg kg−1。使用 31P NMR 光谱，在单酯和二酯区域检测到 11 个 Po 物质。此外，还检测到正磷酸盐、焦磷酸盐和膦酸盐。使用 EH，类植酸磷被鉴定为最常见的酶不稳定磷，其次是不稳定的单酯磷和二酯磷。 NMR和EH数据的比较表明，两种方法测定的样品中主要P形态的分布模式相似（r = 0.712，p < 0.05）。 EH 后对提取物进行的额外 31P NMR 光谱分析显示，与未水解的提取物相比，单酯和焦磷酸区域显着减少，正磷酸信号相应增加。基于这些数量和水解数据，我们提出，植物生物质残留物中磷的循环利用是富营养化湖泊藻类繁殖可用磷长期自我调节的重要机制。
Solution Phosphorus-31 nuclear magnetic resonance (31P NMR) spectroscopy coupled with enzymatic hydrolysis (EH) with commercially available phosphatases was used to characterize phosphorus (P) compounds in extracts of the dominant aquatic macrophytes and algae in a eutrophic lake. Total extractable organic P (Po) concentrations ranged from 504 to 1643 mg kg−1 and 2318 to 8395 mg kg−1 for aquatic macrophytes and algae, respectively. Using 31P NMR spectroscopy, 11 Po species were detected in the mono- and diester region. Additionally, orthophosphate, pyrophosphate and phosphonates were also detected. Using EH, phytate-like P was identified as the prevalent class of enzyme-labile Po, followed by labile monoester- and diester-P. Comparison of the NMR and EH data indicated that the distribution pattern of major P forms in the samples determined by the two methods was similar (r = 0.712, p < 0.05). Additional 31P NMR spectroscopic analysis of extracts following EH showed significant decreases in the monoester and pyrophosphate regions, with a corresponding increase in the orthophosphate signal, as compared to unhydrolyzed extracts. Based on these quantity and hydrolysis data, we proposed that recycling of Po in vegetative biomass residues is an important mechanism for long-term self-regulation of available P for algal blooming in eutrophic lakes.
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影响因子：
2.6
作者：
FEUILLADE, J;BIELICKI, G;RENOU, JP
通讯作者：
RENOU, JP
影响因子：
4.6
作者：
Godinot C;Gaysinski M;Thomas OP;Ferrier-Pagès C;Grover R
通讯作者：
Grover R
影响因子：
2.9
作者：
Doolette, A. L.;Smernik, R. J.;Dougherty, W. J.
通讯作者：
Dougherty, W. J.
影响因子：
9.7
作者：
Jarosch, Klaus A.;Doolette, Ashlea L.;Buenemann, Else K.
通讯作者：
Buenemann, Else K.
通过溶液 P-31 核磁共振波谱表征湖泊大型植物和藻类中的磷形态
影响因子：
5.8
作者：
Feng, Weiying;Zhu, Yuanrong;Fan, Mingle
通讯作者：
Fan, Mingle