Sodium dependency of active chloride transport across isolated fish skin (Gillichthys mirabilis).
Sodium dependency of active chloride transport across isolated fish skin (Gillichthys mirabilis).
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活性氯通过离体鱼皮（Gillichthys mirabilis）运输的钠依赖性。
DOI：
10.1113/jphysiol.1981.sp013899
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Marshall,WS
中科院分区：
文献类型：
--
作者：
Marshall,WS
1.利用海洋硬骨鱼Gillichthys mirabilis的离体皮肤研究了硫氰酸盐、哇巴因、离子取代林格溶液和电化学梯度对Na+和Cl-转运的影响。2.在沐浴溶液中，用胆碱双侧替代Na+可使净Cl-通量减少93%，表明皮肤的主动Cl-转运是Na-依赖性的。3.硫氰酸盐抑制短路电流的ED 50为6.4 x 10（-4）M，在10（-2）M时，Cl-流出、流入、净通量和短路电流分别降低68%、33%、74%和81%。4.哇巴因（10（-5）M）分别使Cl-流出量和净流量减少56%和86%，表明Cl-转运需要Na，K-ATP酶。5.随后向哇巴因处理的皮肤中添加硫氰酸盐会减少氯外流、净通量和短路电流，这表明这两种试剂在参与氯转运的不同部位发挥作用。6.葡萄糖酸盐在丝氨酸侧对Cl-的单侧取代不影响Cl-内流，表明Cl-被动转运是通过Fickean扩散，而不是Cl-Cl交换扩散。7.向粘膜侧添加NaCl（模拟体内海水条件）可增加Cl-内流和跨上皮电位，并降低组织阻力。Cl-与高渗盐水在粘膜侧的净流量（分泌）（0.51 +/-0.06 muequiv/cm 2. hr）表明皮肤在体内可分泌Cl-。8.通过皮肤的Na+通量是被动的，因为在闭路和开路条件下观察到的通量比（流出/流入）与Ussing-Teorell方程预测的相似。9.渗透率比（PNa：PCl）约为5.4：1.0，表明皮肤对Na+更具渗透性，并且至少部分浆膜阳性跨上皮电位可能是Na+扩散电位。10.结果表明，鳃鱼皮肤分泌Cl-是Na，K-ATP酶和丝氨酸Na+参与的次级主动转运。
1. The effects of thiocyanate, ouabain, ion‐substituted Ringer solution and electrochemical gradients on Na+ and Cl‐ transport were examined using the isolated skin of the marine teleost, Gillichthys mirabilis. 2. Bilateral replacement of Na+ with choline in the bathing solutions reduces net Cl‐ flux by 93%, indicating that active Cl‐ transport by the skin is Na‐dependent. 3. Thiocyanate inhibits short‐circuit current with an ED50 of 6.4 x 10(‐4)M, and, at 10(‐2)M, decreases Cl‐efflux, influx, net flux and short‐circuit current by 68, 33, 74 and 81%, respectively. 4. Ouabain (10(‐5)M) reduces Cl‐ efflux and net flux by 56 and 86%, respectively, indicating that the Cl‐ transport requires Na,K‐ATPase. 5. Subsequent addition of thiocyanate to ouabain‐treated skin reduces Cl‐ efflux, net flux and short‐circuit current, suggesting that the two agents operate at different sites involved in Cl‐ transport. 6. Unilateral substitution of gluconate for Cl‐ on the serosal side does not affect Cl‐ influx, indicating that Cl‐ passive transport is via Fickean diffusion, not Cl‐Cl exchange diffusion. 7. The addition of NaCl to the mucosal side, which mimics the in vivo sea‐water condition, increases Cl‐ influx and transepithelial potential and decreases tissue resistance. The net flux (secretion) of Cl‐ with hypertonic saline on the mucosal side (0.51 +/‐ 0.06 muequiv/cm2 . hr) demonstrates that the skin could secrete Cl‐ in vivo. 8. Na+ fluxes across the skin are passive, as the observed flux ration (efflux/influx) is similar to that predicted by the Ussing‐Teorell equation under both closed‐ and open‐circuit conditions. 9. The permeability ratio (PNa:PCl) in approximately 5.4:1.0, indicating that the skin is more permeable to Na+, and that at least part of the serosa‐positive transepithelial potential may be a Na+ diffusion potential. 10. The results suggest that Cl‐ secretion by Gillichthys skin is secondary active transport involving Na,K‐ATPase and serosal Na+.
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DOI：
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DOI：
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