Collagen studies in newborn rat kidneys with incomplete ureteric obstruction.
Collagen studies in newborn rat kidneys with incomplete ureteric obstruction.
复制标题
输尿管不完全梗阻新生大鼠肾脏的胶原蛋白研究。
作者：
A. Haralambous;D. Chan;R. Walker;H. Powell;G. Becker;C. Jones
对患有不完全输尿管梗阻的新生大鼠进行胶原蛋白研究，以描述和量化早期发育年龄时尿路梗阻引起的胶原蛋白沉积变化。通过将左侧输尿管置于由腰大肌形成的隧道中，在三日龄大鼠中制造不完全输尿管梗阻，假手术对照组进行剖腹手术。在第10、17、24或31天处死大鼠。胶原蛋白I，III，IV和V型进行了本地化的间接免疫荧光显微镜，肾脏的总胶原蛋白含量进行定量使用羟脯氨酸分析，和胶原蛋白I型和III型进行定量使用溴化氰（CNBr）肽分析。所有胶原的免疫荧光染色增加被发现在弥漫性增宽的肾髓质，更集中在皮质髓质。胶原蛋白I，III和V型，但不是胶原蛋白IV型，也被发现在带状的皮质与髓质交界处的髓质。IV型胶原染色增加，发现在增厚和曲折的肾小管基底膜（TBM）的梗阻肾。在24和31日龄时，阻塞肾脏的总胶原含量与对侧肾脏和来自假手术对照的肾脏中的量相比显著增加（在每种情况下，分别为P < 0.01，Wilcoxon配对秩和检验和Mann Whitney U-检验）。肾组织胶原含量与肾积水程度相关（斯皮尔曼相关检验，r = 0.78，P < 0.02）。CNBr肽分析表明，正常新生大鼠肾脏中超过50%的胶原蛋白是I型胶原蛋白，约25%是III型胶原蛋白。在阻塞的肾脏中，大部分胶原蛋白也是I型胶原蛋白和III型胶原蛋白，尽管与对照组相比，这些胶原蛋白类型所占的总胶原蛋白的比例降低。与对照组相比，对侧肾脏中III型胶原的量减少。因此，新生儿肾脏对梗阻的反应导致肾小管和TBM中一系列胶原的量增加，这种反应的程度部分与肾积水的程度有关。
Collagen studies in newborn rats with incomplete ureteric obstruction were performed to describe and quantify changes in collagen deposition resulting from urinary tract obstruction at an early developmental age. Incomplete ureteric obstruction was created in three-day-old rats by placing the left ureter in a tunnel formed by the psoas muscle, and sham-operated controls underwent a laparotomy. The rats were sacrificed at 10, 17, 24 or 31 days. Collagen types I, III, IV, and V were localized by indirect immunofluorescence microscopy, the total collagen content of the kidney was quantitated using hydroxyproline analysis, and collagen types I and III were quantitated using cyanogen bromide (CNBr) peptide analysis. Increased immunofluorescent staining for all of the collagens was found in the diffusely widened medullary interstitium of the obstructed kidney, and more focally in the cortical interstitium. Collagen types I, III and V, but not collagen type IV, were also found in bands in the interstitium at the junction of the cortex with the medulla. Increased staining for collagen type IV was found in thickened and tortuous tubular basement membranes (TBM) of the obstructed kidneys. The total collagen content of the obstructed kidney was significantly increased compared to the amounts in both the contralateral kidneys and in the kidneys from sham-operated controls at 24 and 31 days of age (P < 0.01 in each case, Wilcoxon matched pairs rank sum test and Mann Whitney U-test, respectively). The amount of collagen in the kidneys correlated with the degree of hydronephrosis (Spearman correlation test, r = 0.78, P < 0.02). CNBr peptide analysis demonstrated that over 50% of the collagen in the normal neonatal rat kidney was collagen type I and approximately 25% was collagen type III. In the obstructed kidneys most of the collagen was also collagen type I and collagen type III, although the proportion of total collagen comprised by these collagen types was decreased compared with the controls. The amount of collagen type III in the contralateral kidneys was reduced compared to that in the controls. Thus, the neonatal renal response to obstruction resulted in increased amounts of a range of collagens in the interstitium and TBM, and the extent of this response was partially related to the degree of hydronephrosis.
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DOI：
10.1172/jci114816
发表时间：
1990-10
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
K. Harris;J. Lefkowith;S. Klahr;G. Schreiner
通讯作者：
K. Harris;J. Lefkowith;S. Klahr;G. Schreiner
必需脂肪酸缺乏减少了兔肾积水中炎症细胞的侵袭，从而抑制了类二十烷酸的过量产生。
DOI：
--
发表时间：
1988
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Spaethe,SM;Freed,MS;DeSchryver-Kecskemeti,K;Lefkowith,JB;Needleman,P
通讯作者：
Needleman,P
影响因子：
19.6
作者：
SCHREINER, GF;HARRIS, KPG;KLAHR, S
通讯作者：
KLAHR, S
白细胞去除对大鼠梗阻后肾功能的影响。
影响因子：
19.6
作者：
Harris,KP;Schreiner,GF;Klahr,S
通讯作者：
Klahr,S