Growth is required for perception of water availability to pattern root branches in plants.
Growth is required for perception of water availability to pattern root branches in plants.
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DOI：
10.1073/pnas.1710709115
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发表时间：
2018-01-23
影响因子：
11.1
通讯作者：
Dinneny JR
中科院分区：
文献类型：
--
作者：
Robbins NE 2nd;Dinneny JR
植物的根激活侧向分支以响应与可用水的接触，但这种环境信号被感知的机制尚不清楚。通过经验和数学建模方法的结合，我们发现了组织生长在这一过程中的核心作用。生长引起水分吸收，在这一过程中发生的生物物理变化被生物体解释为定位新的侧枝。这一观察结果在我们理解环境如何影响植物发育方面取得了重大进展，并证明了对水的感知与根的核心生物功能密切相关。水分可利用率是植物发育的有效调节剂，并通过称为水模式的过程诱导根分支。水模式使根能够将侧枝定位在水分充足的区域，如潮湿的土壤或琼脂培养基，同时防止它们在水分不足的地方出现，如空气中。在水型化过程中，根系感知水的空间分布的机制尚不清楚。利用玉米的初生根，我们发现水分模式的发育能力仅限于根尖的生长区域。过去的研究表明，当可用水分布不对称时，生长会在整个器官中产生水势梯度。利用数学模型，我们预测在水模式的能力区也会产生大量持续生长的水势梯度，并且这些生物物理线索会为侧根的模式提供信息。采用不同的化学和环境处理，我们实验证明生长是必要的正常水化侧根。组织对潮湿表面或空气的局部反应的转录组学特征揭示了信号和生理途径的广泛调节，其中一些是生长依赖性的。我们的工作支持一种控制水模式的“生长感知”机制，通过这种机制，水的可用性线索可以通过持续生长的水运动来解释。
Plant roots activate lateral branching in response to contact with available water, but the mechanism by which this environmental signal is perceived is poorly understood. Through a combination of empirical and mathematical-modeling approaches we discovered a central role of tissue growth in this process. Growth causes water uptake, and the biophysical changes that occur during this process are interpreted by the organism to position new lateral branches. This observation is a significant advancement in our understanding of how the environment shapes plant development and demonstrates that perception of water is intimately tied to a core biological function of the root. Water availability is a potent regulator of plant development and induces root branching through a process termed hydropatterning. Hydropatterning enables roots to position lateral branches toward regions of high water availability, such as wet soil or agar media, while preventing their emergence where water is less available, such as in air. The mechanism by which roots perceive the spatial distribution of water during hydropatterning is unknown. Using primary roots of Zea mays (maize) we reveal that developmental competence for hydropatterning is limited to the growth zone of the root tip. Past work has shown that growth generates gradients in water potential across an organ when asymmetries exist in the distribution of available water. Using mathematical modeling, we predict that substantial growth-sustained water potential gradients are also generated in the hydropatterning competent zone and that such biophysical cues inform the patterning of lateral roots. Using diverse chemical and environmental treatments we experimentally demonstrate that growth is necessary for normal hydropatterning of lateral roots. Transcriptomic characterization of the local response of tissues to a moist surface or air revealed extensive regulation of signaling and physiological pathways, some of which we show are growth-dependent. Our work supports a “sense-by-growth” mechanism governing hydropatterning, by which water availability cues are rendered interpretable through growth-sustained water movement.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
3
作者：
ERICKSON, RO;SILK, WK
通讯作者：
SILK, WK
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影响因子：
14.9
作者：
Du Z;Zhou X;Ling Y;Zhang Z;Su Z
通讯作者：
Su Z
DOI：
10.1016/0304-4211(81)90103-6
发表时间：
1981-01-01
期刊：
PLANT SCIENCE LETTERS
影响因子：
--
作者：
COLOMBO, R;BONETTI, A;LADO, P
通讯作者：
LADO, P
影响因子：
3.4
作者：
Ibata, Keiji;Takimoto, Shinichi;Yasui, Masato
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Yasui, Masato