Dynamic biofilm architecture confers individual and collective mechanisms of viral protection.
Dynamic biofilm architecture confers individual and collective mechanisms of viral protection.
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DOI：
10.1038/s41564-017-0050-1
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发表时间：
2018-01
影响因子：
28.3
通讯作者：
Drescher K
中科院分区：
文献类型：
--
作者：
Vidakovic L;Singh PK;Hartmann R;Nadell CD;Drescher K
在自然界中，细菌主要生活在表面附着的多细胞群落中，称为生物膜; 1-6;。在医疗环境中，生物膜在慢性和急性感染期间造成毁灭性损害;事实上，细菌通常被视为人类疾病的病原体; 7。然而，细菌本身也会遭受疾病，最明显的是以病毒病原体的形式出现的噬菌体; 8-12;，它们是地球上最丰富的复制实体。毫无疑问，噬菌体生物膜的遭遇在环境中很常见，但决定这些遭遇结果的机制尚不清楚。使用大肠杆菌生物膜和裂解性噬菌体T7作为模型，我们发现CsgA（卷曲聚合物）的淀粉样蛋白纤维网络通过两种不同的机制保护生物膜免受噬菌体攻击。首先，集体细胞保护的结果，从抑制噬菌体运输到生物膜，我们证明在体内和体外。其次，CsgA纤维通过覆盖细胞表面并结合噬菌体颗粒来保护细胞，从而防止它们附着在细胞外部。这些见解生物膜噬菌体相互作用的噬菌体应用在生物技术，噬菌体治疗和进化动力学的噬菌体与其细菌宿主的设计具有广泛的影响。
In nature, bacteria primarily live in surface-attached, multicellular communities, termed biofilms; 1-6;. In medical settings, biofilms cause devastating damage during chronic and acute infections; indeed, bacteria are often viewed as agents of human disease; 7;. However, bacteria themselves suffer from diseases, most notably in the form of viral pathogens termed bacteriophages; 8-12;, which are the most abundant replicating entities on Earth. Phage-biofilm encounters are undoubtedly common in the environment, but the mechanisms that determine the outcome of these encounters are unknown. Using Escherichia coli biofilms and the lytic phage T7 as models, we discovered that an amyloid fibre network of CsgA (curli polymer) protects biofilms against phage attack via two separate mechanisms. First, collective cell protection results from inhibition of phage transport into the biofilm, which we demonstrate in vivo and in vitro. Second, CsgA fibres protect cells individually by coating their surface and binding phage particles, thereby preventing their attachment to the cell exterior. These insights into biofilm-phage interactions have broad-ranging implications for the design of phage applications in biotechnology, phage therapy and the evolutionary dynamics of phages with their bacterial hosts.
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影响因子：
6.4
作者：
Serra DO;Richter AM;Klauck G;Mika F;Hengge R
通讯作者：
Hengge R
DOI：
10.1126/science.1231887
发表时间：
2013-02-01
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hu B;Margolin W;Molineux IJ;Liu J
通讯作者：
Liu J
DOI：
10.1038/nrmicro2853
发表时间：
2012-09
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1126/science.1222981
发表时间：
2012-07-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Berk V;Fong JC;Dempsey GT;Develioglu ON;Zhuang X;Liphardt J;Yildiz FH;Chu S
通讯作者：
Chu S
影响因子：
64.8
作者：
Forde, Samantha E.;Beardmore, Robert E.;Hurst, Laurence D.
通讯作者：
Hurst, Laurence D.