Confirmation of inhibitingTLR4/MyD88/NF-κB Signalling Pathway by Duhuo Jisheng Decoction on Osteoarthritis: A Network Pharmacology Approach-Integrated Experimental Study.
Confirmation of inhibitingTLR4/MyD88/NF-κB Signalling Pathway by Duhuo Jisheng Decoction on Osteoarthritis: A Network Pharmacology Approach-Integrated Experimental Study.
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独活寄生汤抑制TLR4/MYD88/NF-κB信号通路的网络药理学研究--综合实验研究
DOI：
10.3389/fphar.2021.784822
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发表时间：
2021
影响因子：
5.6
通讯作者：
Ye H
中科院分区：
文献类型：
--
作者：
Liu L;Xu L;Wang S;Wang L;Wang X;Xu H;Li X;Ye H
本研究通过网络药理学方法（network pharmacology approach， NPA）整合实验，探讨TLR4/MyD88/NF-κB信号通路是否在独活生汤（DHJSD）治疗骨关节炎（OA）中发挥重要作用。两项实验分别进行：利用6个oa相关基因序列对DHJSD进行NPA，筛选关键通路。NPA发现TLR4/MyD88/NF-κB信号通路在DHJSD治疗OA中起重要作用，传统的western blot分析和qPCR证实了这一点。此外，DHJSD可恢复细胞模型中miR-146a-5p和miR-34a-5p的变化，从而协同促进OA治疗。toll样受体信号通路和NF-κB信号通路被mirna调控的基因通路显著富集。本研究发现并证实了TLR4/MyD88/NF-κB信号通路是DHJSD基础OA治疗中必不可少的炎症信号通路。本研究结果为进一步评价该药治疗OA疗效的调控机制提供了依据。
This study was conducted to identify whether the TLR4/MyD88/NF-κB signalling pathway plays a vital role in osteoarthritis (OA) treatment with Duhuo Jisheng Decoction (DHJSD) on the basis of a network pharmacology approach (NPA)-integrated experiment. Two experiments were conducted as follow: NPA for DHJSD using six OA-related gene series and the key pathway was screened out using NPA. NPA identified a vital role for the TLR4/MyD88/NF-κB signalling pathway in OA treatment with DHJSD, the conventional western blot analysis and qPCR confirmed it. Furthermore, changes of miR-146a-5p and miR-34a-5p in the cellular models were recovered by DHJSD administration, which synergistically contributed to OA therapy. The toll-like receptor signalling pathway and the NF-κB signalling pathway were meaningfully enriched by the miRNA-regulated gene pathways. This study identified and confirmed the TLR4/MyD88/NF-κB signalling pathway is an essential inflammatory signalling pathway in the DHJSD underlying OA treatment. The results provide a basis for further evaluation of the regulatory mechanism of the drug’s efficacy in treating OA.
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DOI：
10.1002/art.41552
发表时间：
2021-03
期刊：
Arthritis & rheumatology (Hoboken, N.J.)
影响因子：
--
作者：
Endisha H;Datta P;Sharma A;Nakamura S;Rossomacha E;Younan C;Ali SA;Tavallaee G;Lively S;Potla P;Shestopaloff K;Rockel JS;Krawetz R;Mahomed NN;Jurisica I;Gandhi R;Kapoor M
通讯作者：
Kapoor M
DOI：
10.1155/2016/2960372
发表时间：
2016
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Liu H;Zeng L;Yang K;Zhang G
通讯作者：
Zhang G
DOI：
10.1155/2017/5190986
发表时间：
2017
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Li L;Liu H;Shi W;Liu H;Yang J;Xu D;Huang H;Wu L
通讯作者：
Wu L
影响因子：
5.4
作者：
Lin P;Weng X;Liu F;Ma Y;Chen H;Shao X;Zheng W;Liu X;Ye H;Li X
通讯作者：
Li X
影响因子：
4
作者：
Honsawek, Sittisak;Malila, Somkiat;Parvizi, Javad
通讯作者：
Parvizi, Javad