Effect of immunization with lutropin-receptor on the ovarian function of rabbits.

Effect of immunization with lutropin-receptor on the ovarian function of rabbits.
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促黄体素受体免疫对家兔卵巢功能的影响。

DOI:
10.1080/15321819508013546
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发表时间:
1995
期刊:
Journal of immunoassay.
影响因子:
--
通讯作者:
Saxena,BB
Saxena,BB
中科院分区:
--
文献类型:
--
作者:
Singh,M;Lin,SQ;Saxena,BB

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对三只新西兰白色成年雌性家兔(指定为A、B和C)进行免疫接种,以产生促黄体激素受体抗体。抗血清可抑制125 I-hCG与促黄体激素受体的结合及hCG刺激的大鼠睾丸间质细胞产生睾酮。在研究期间,末次免疫接种后约10个月,家兔对注射75 IU人绒毛膜促性腺激素或剖腹手术显示的交配无排卵反应。随着抗体滴度下降,诱导排卵和剖腹手术显示排卵和黄体形成恢复。然而,当兔子A和B交配并人工授精时,没有发生妊娠。这些观察结果表明促黄体激素受体抗体可导致雌兔不育。
Three New Zealand white adult female rabbits, designated as A, B, and C, were immunized to produce lutropin receptor antibodies. Antisera inhibited the binding of125I-hCG to the lutropin receptor and the production of testosterone by hCG stimulated rat Leydig cells. During the study period of, approximately 10 months after the last immunization, rabbits did not ovulate in response to an injection of 75 IU of human chorionic gonadotropin or mating as revealed by laparotomy. As the antibody titers declined, induction of ovulation and laparotomy revealed restoration of ovulation and corpus luteum formation. However, no pregnancy occurred when rabbits A and B were mated and artificially inseminated. These observations indicate that lutropin receptor antibodies can cause infertility in female rabbits.
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发表时间: 1973
期刊: The Journal of Clinical Endocrinology and Metabolism
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