Three-dimensional cellular ultrastructure resolved by X-ray microscopy.

Three-dimensional cellular ultrastructure resolved by X-ray microscopy.
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DOI:
10.1038/nmeth.1533
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发表时间:
2010-12
期刊:
影响因子:
48
通讯作者:
McNally JG
McNally JG
中科院分区:
生物学1区
文献类型:
--
作者:
Schneider G;Guttmann P;Heim S;Rehbein S;Mueller F;Nagashima K;Heymann JB;Müller WG;McNally JG

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We developed an X-ray microscope using partially coherent object illumination instead of previously used quasi-incoherent illumination. The design permitted the incorporation of a cryogenic tilt stage, enabling tomography of frozen-hydrated, intact adherent cells. We obtained three-dimensional reconstructions of mouse adenocarcinoma cells at ~36-nm (Rayleigh) and ~70-nm (Fourier ring correlation) resolution, which allowed us to visualize the double nuclear membrane, nuclear pores, nuclear membrane channels, mitochondrial cristae and lysosomal inclusions.
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