Three-dimensional cellular ultrastructure resolved by X-ray microscopy.
Three-dimensional cellular ultrastructure resolved by X-ray microscopy.
复制标题
DOI:
10.1038/nmeth.1533
复制
发表时间:
2010-12
期刊:
影响因子:
48
通讯作者:
McNally JG
中科院分区:
文献类型:
--
作者:
Schneider G;Guttmann P;Heim S;Rehbein S;Mueller F;Nagashima K;Heymann JB;Müller WG;McNally JG
We developed an X-ray microscope using partially coherent object illumination instead of previously used quasi-incoherent illumination. The design permitted the incorporation of a cryogenic tilt stage, enabling tomography of frozen-hydrated, intact adherent cells. We obtained three-dimensional reconstructions of mouse adenocarcinoma cells at ~36-nm (Rayleigh) and ~70-nm (Fourier ring correlation) resolution, which allowed us to visualize the double nuclear membrane, nuclear pores, nuclear membrane channels, mitochondrial cristae and lysosomal inclusions.
登录
查看更多内容
DOI:
10.1083/jcb.136.3.531
发表时间:
1997-02-10
期刊:
The Journal of cell biology
影响因子:
--
作者:
Fricker M;Hollinshead M;White N;Vaux D
通讯作者:
Vaux D
影响因子:
3.3
作者:
Larabell, CA;Le Gros, MA
通讯作者:
Le Gros, MA
影响因子:
2.2
作者:
Schneider, G
通讯作者:
Schneider, G
影响因子:
2.2
作者:
Weiss, D;Schneider, G;Schmahl, G
通讯作者:
Schmahl, G
影响因子:
3.8
作者:
Bertilson, Michael;von Hofsten, Olov;Hertz, Hans M.
通讯作者:
Hertz, Hans M.