Targeted non-covalent self-assembled nanoparticles based on human serum albumin.
Targeted non-covalent self-assembled nanoparticles based on human serum albumin.
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DOI：
10.1016/j.biomaterials.2011.10.005
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发表时间：
2012-01
期刊：
影响因子：
14
通讯作者：
van Leeuwen, Fijs W. B.
中科院分区：
文献类型：
--
作者：
Bunschoten, Anton;Buckle, Tessa;Kuil, Joeri;Luker, Gary D.;Luker, Kathryn E.;Nieweg, Omgo E.;van Leeuwen, Fijs W. B.
人血清白蛋白（HSA）是一种生物纳米载体，它与许多合成分子和生物分子形成非共价复合物。先前我们证明，放射性标记的基于HSA的纳米粒子可与荧光菁染料形成非共价复合物，产生用于肿瘤引流淋巴结手术导航的成像剂。在此，自组装方法实现了快速的临床转化。基于这一经验，我们推断将这种非共价技术扩展到靶向方法会很有趣。我们探索了HSA通过近红外（NIR）菁染料与肽形成非共价自组装复合物的能力。利用HSA - Cy5与非共价结合的荧光分子吲哚菁绿（ICG）、IR783 - CO₂H以及三种IR783标记的靶向肽之间的福斯特共振能量转移（FRET）猝灭相互作用来监测复合物的组装和拆卸。HSA与IR783标记的肽之间的主 - 客体相互作用使得能够形成（生物）纳米粒子，其表面覆盖有可能靶向αvβ3 - 整合素、趋化因子受体4（CXCR4）和生长抑素受体的肽。展示了CXCR4靶向（生物）纳米粒子在前哨淋巴结手术中的潜力。通过将近红外染料标记的肽非共价结合到一种已获临床批准的HSA支架上，我们很容易地形成了靶向生物纳米粒子。
Human serum albumin (HSA) is a biological nanocarrier that forms non-covalent complexes with a number of synthetic and biomolecules. Previously we demonstrated radiolabeled HSA-based nanoparticles can form non-covalent complexes with fluorescent cyanine dyes yielding imaging agents for surgical guidance towards tumor draining lymph nodes. Here the self-assembly approach enabled rapid clinical translation. Based on this experience we reasoned it would be interesting to expand this non-covalent technology to a targeted approach. The ability of HSA to form non-covalent self-assembled complexes with peptides via near-infrared (NIR) cyanine dyes was explored. Föster resonance energy transfer (FRET) quenching interactions between HSA-Cy5 and the non-covalently bound fluorescent molecules indocyanine green (ICG), IR783-CO2H and three IR783-labeled targeting peptides were used to monitor complex assembly and disassembly. The host-guest interactions between HSA and IR783-labeled peptides enabled the formation of (bio)nanoparticles that are coated with peptides that may target αvβ3-integrins, the chemokine receptor 4 (CXCR4), and somatostatin receptors. The potential of CXCR4-targeted (bio)nanoparticles in sentinel lymph node procedures is demonstrated. By non-covalently binding NIR-dye labeled peptides to an already clinically approved HSA-scaffold, we have readily formed targeted bionanoparticles.
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影响因子：
2.9
作者：
Berezin MY;Guo K;Akers W;Livingston J;Solomon M;Lee H;Liang K;Agee A;Achilefu S
通讯作者：
Achilefu S
影响因子：
2.5
作者：
Schaafsma, Boudewijn E.;Mieog, J. Sven D.;Hutteman, Merlijn;Van der Vorst, Joost R.;Kuppen, Peter J. K.;Lowik, Clemens W. G. M.;Frangioni, John V.;Van de Velde, Cornelis J. H.;Vahrmeijer, Alexander L.
通讯作者：
Vahrmeijer, Alexander L.
影响因子：
4
作者：
Meincke, Manuela;Tiwari, Sanjay;Hattermann, Kirsten;Kalthoff, Holger;Mentlein, Rolf
通讯作者：
Mentlein, Rolf
DOI：
10.1073/pnas.0506440102
发表时间：
2005-12-13
影响因子：
11.1
作者：
Simard, JR;Zunszain, PA;Hamilton, JA
通讯作者：
Hamilton, JA
影响因子：
46.9
作者：
Becker, A;Hessenius, C;Grötzinger, C
通讯作者：
Grötzinger, C