Identification of drought stress-responsive transcription factors in ramie (Boehmeria nivea L. Gaud).
Identification of drought stress-responsive transcription factors in ramie (Boehmeria nivea L. Gaud).
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苎麻 (Boehmeria nivea L. Gaud) 干旱胁迫响应转录因子的鉴定
DOI：
10.1186/1471-2229-13-130
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发表时间：
2013-09-10
影响因子：
5.3
通讯作者：
Tang S
中科院分区：
文献类型：
--
作者：
Liu T;Zhu S;Tang Q;Yu Y;Tang S
研究背景苎麻纤维是从苎麻茎皮中提取的一种重要的天然纤维。干旱是苎麻生长发育受到严重抑制、纤维产量下降的主要环境胁迫。结果利用Illumina测序技术，从干旱胁迫苎麻（DS）和对照苎麻（CO）的cDNA文库中分别获得了约480万和470万个（M）21-nt的cDNA标签。将这两个文库中的标签与苎麻转录组进行比对，分别得到23，912个和22，826个苎麻基因与DS文库和CO文库中的标签进行匹配。根据标签频率的差异比较CO和DS苎麻的基因表达水平，发现有1516个潜在的干旱胁迫响应基因，其中24个基因起转录因子（TF）的作用。在这24个转录因子中，编码赤霉素（GAs）信号通路中关键负调控因子DELLA蛋白的unigene 19721在水分胁迫下表达显著上调，从而增加了DS文库中的标签丰度，这可能是干旱胁迫抑制苎麻生长的原因。为了验证这些潜在的逆境响应转录因子在水分亏缺条件下的表达变化，本研究选取unigene 19721和另外11个潜在的逆境响应转录因子，采用实时荧光定量PCR（qRT-PCR）技术对这些转录因子在充分供水和干旱胁迫条件下的表达进行了分析，结果表明，所有12个转录因子均真实地参与了干旱胁迫的响应。利用Illumina tag-sequencing和qRT-PCR技术首次在苎麻中发现了12个参与干旱胁迫响应的转录因子。这些干旱胁迫响应转录因子的发现将有助于进一步了解苎麻的干旱胁迫调控机制，提高苎麻的耐旱能力。
BackgroundRamie fiber extracted from stem bark is one of the most important natural fibers. Drought is a main environment stress which severely inhibits the stem growth of ramie and leads to a decrease of the fiber yield. The drought stress-regulatory mechanism of ramie is poorly understood.ResultUsing Illumina sequencing, approximately 4.8 and 4.7 million (M) 21-nt cDNA tags were respectively sequenced in the cDNA libraries derived from the drought-stressed ramie (DS) and the control ramie under well water condition (CO). The tags generated from the two libraries were aligned with ramie transcriptome to annotate their function and a total of 23,912 and 22,826 ramie genes were matched by these tags of DS and CO library, respectively. Comparison of gene expression level between CO and DS ramie based on the differences of tag frequencies appearing in the two libraries revealed that there were 1516 potential drought stress-responsive genes, in which 24 genes function as transcription factor (TF). Among these 24 TFs, the unigene19721 encoding the DELLA protein which is a key negative regulator in gibberellins (GAs) signal pathway was probably markedly up-regulated under water stress for a increase of tag abundance in DS library, which is possibly responsible for the inhibition of the growth of drought-stressed ramie. In order to validate the change of expression of these potential stress-responsive TFs under water deficit condition, the unigene19721 and another eleven potential stress-responsive TFs were chosen for further expression analysis in well-watered and drought-stressed ramie by real-time quantitative PCR (qRT-PCR) and the result showed that all 12 TFs were authentically involved in the response of drought stress.ConclusionIn this study, twelve TFs involving in the response of drought stress were first found by Illumina tag-sequencing and qRT-PCR in ramie. The discovery of these drought stress-responsive TFs will be helpful for further understanding the drought stress-regulatory mechanism of ramie and improving the drought tolerance ability of ramie.
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影响因子：
2.8
作者：
Huang YW;Huang TH;Wang LS
通讯作者：
Wang LS
影响因子：
4.4
作者：
Aprile A;Mastrangelo AM;De Leonardis AM;Galiba G;Roncaglia E;Ferrari F;De Bellis L;Turchi L;Giuliano G;Cattivelli L
通讯作者：
Cattivelli L
影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
7.4
作者：
Hauvermale, Amber L.;Ariizumi, Tohru;Steber, Camille M.
通讯作者：
Steber, Camille M.
影响因子：
4.4
作者：
Meyer E;Aglyamova GV;Wang S;Buchanan-Carter J;Abrego D;Colbourne JK;Willis BL;Matz MV
通讯作者：
Matz MV