Advances and perspectives in discovery and functional analysis of small secreted proteins in plants.
Advances and perspectives in discovery and functional analysis of small secreted proteins in plants.
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DOI：
10.1038/s41438-021-00570-7
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发表时间：
2021-06-01
影响因子：
8.7
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Hu XL;Lu H;Hassan MM;Zhang J;Yuan G;Abraham PE;Shrestha HK;Villalobos Solis MI;Chen JG;Tschaplinski TJ;Doktycz MJ;Tuskan GA;Cheng ZM;Yang X
小分泌蛋白（SSP）长度小于250个氨基酸，并通过常规蛋白分泌途径或非常规蛋白分泌途径主动转运出细胞。在植物中，SSPs已被发现在各种过程中发挥重要作用，包括植物生长和发育，植物对非生物和生物胁迫的响应，以及有益的植物-微生物相互作用。在过去的10年中，在与农业，生物能源和园艺相关的几种植物物种中的SSPs的鉴定和功能表征方面取得了实质性进展。然而，在植物基因组中可能存在大量尚未被发现的SSP，这在很大程度上是由于现有计算算法的限制。基因组学、转录组学和蛋白质组学研究的最新进展，以及基于机器学习的新计算算法的发展，为植物中新型SSP的全基因组发现提供了前所未有的能力。本文综述了近年来已知的SSPs及其在不同植物中的功能。然后，我们提供了一个更新的计算和实验方法，可用于发现新的SSP。最后，我们讨论了阐明植物中SSP的生物学功能的策略。
Small secreted proteins (SSPs) are less than 250 amino acids in length and are actively transported out of cells through conventional protein secretion pathways or unconventional protein secretion pathways. In plants, SSPs have been found to play important roles in various processes, including plant growth and development, plant response to abiotic and biotic stresses, and beneficial plant–microbe interactions. Over the past 10 years, substantial progress has been made in the identification and functional characterization of SSPs in several plant species relevant to agriculture, bioenergy, and horticulture. Yet, there are potentially a lot of SSPs that have not been discovered in plant genomes, which is largely due to limitations of existing computational algorithms. Recent advances in genomics, transcriptomics, and proteomics research, as well as the development of new computational algorithms based on machine learning, provide unprecedented capabilities for genome-wide discovery of novel SSPs in plants. In this review, we summarize known SSPs and their functions in various plant species. Then we provide an update on the computational and experimental approaches that can be used to discover new SSPs. Finally, we discuss strategies for elucidating the biological functions of SSPs in plants.
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