Electrochemical immunosensor detection of urinary lactoferrin in clinical samples for urinary tract infection diagnosis.
Electrochemical immunosensor detection of urinary lactoferrin in clinical samples for urinary tract infection diagnosis.
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DOI：
10.1016/j.bios.2010.07.002
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发表时间：
2010-10-15
影响因子：
12.6
通讯作者：
Liao JC
中科院分区：
文献类型：
--
作者：
Pan Y;Sonn GA;Sin ML;Mach KE;Shih MC;Gau V;Wong PK;Liao JC
尿液是所有体液中最丰富和最容易获得的，并且为人类疾病，特别是泌尿道疾病的非侵入性诊断提供了理想的途径。电化学生物传感器由于其优异的灵敏度、低成本和检测包括核酸和蛋白质生物标志物在内的多种靶分子的能力而非常适合于泌尿诊断。我们报告了一种电化学免疫传感器的发展，用于直接检测尿路感染（UTI）的生物标志物乳铁蛋白从感染的临床样本。采用硫醇盐自组装膜（SAM）作为电化学传感器阵列。采用电化学阻抗谱对11-巯基十一酸和6-巯基-1-己醇的混合SAM进行了表征。建立了一种检测尿液中乳铁蛋白的夹心安培免疫分析法，其检测限为145 pg/ml。我们在111例患者来源的尿液样本中验证了乳铁蛋白作为脓尿（尿液中存在白色血细胞）的生物标志物，脓尿是UTI的重要标志。最后，我们证明了多重检测的尿液病原体和乳铁蛋白，通过同时检测细菌核酸（16 S rRNA）和宿主免疫反应蛋白（乳铁蛋白）在一个单一的传感器阵列。我们的研究结果代表了第一个能够定量病原体识别和测量宿主免疫反应的集成传感器平台，可能提供比当前标准不仅更迅速而且信息量更大的临床诊断。
Urine is the most abundant and easily accessible of all body fluids and provides an ideal route for non-invasive diagnosis of human diseases, particularly of the urinary tract. Electrochemical biosensors are well suited for urinary diagnostics due to their excellent sensitivity, low cost, and ability to detect a wide variety of target molecules including nucleic acids and protein biomarkers. We report the development of an electrochemical immunosensor for direct detection of the urinary tract infection (UTI) biomarker lactoferrin from infected clinical samples. An electrochemical biosensor array with alkanethiolate self-assembled monolayer (SAM) was used. Electrochemical impedance spectroscopy was used to characterize the mixed SAM, consisted of 11-mercaptoundecanoic acid and 6-mercapto-1-hexanol. A sandwich amperometric immunoassay was developed for detection of lactoferrin from urine, with a detection limit of 145 pg/ml. We validated lactoferrin as a biomarker of pyuria (presence of white blood cells in urine), an important hallmark of UTI, in 111 patient-derived urine samples. Finally, we demonstrated multiplex detection of urinary pathogens and lactoferrin through simultaneous detection of bacterial nucleic acid (16S rRNA) and host immune-response protein (lactoferrin) on a single sensor array. Our results represent first integrated sensor platform capable of quantitative pathogen identification and measurement of host immune response, potentially providing clinical diagnosis that is not only more expeditious but more informative than the current standard.
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影响因子：
12.6
作者：
Wang, J
通讯作者：
Wang, J
影响因子：
9.4
作者：
Liao, JC;Mastali, M;Haake, DA
通讯作者：
Haake, DA
影响因子：
9.4
作者：
Arao, S;Matsuura, S;Nakanishi, H
通讯作者：
Nakanishi, H
影响因子：
6.6
作者：
Griebling, TL
通讯作者：
Griebling, TL
影响因子：
4.2
作者：
Wang, J
通讯作者：
Wang, J