Compartmentalization of electrophoretically separated analytes in a multiphase microfluidic platform.

Compartmentalization of electrophoretically separated analytes in a multiphase microfluidic platform.
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多相微流体平台中电泳分离分析物的区室化。

DOI:
10.1021/ac301141x
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发表时间:
2012
影响因子:
7.4
通讯作者:
Draper MC
Draper MC
中科院分区:
化学1区
文献类型:
--
作者:
Draper MC

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Herein, we describe the monolithic integration of a multiphase microfluidic system to a microcapillary gel electrophoresis (μCGE) architecture for the complete isolation and storage of separated analyte bands. Within this platform, analyte molecules are separated using microchannel gel electrophoresis, and the eluted bands are stored in a sequence of approximately 40–600 encapsulating microdroplets. Importantly, employing such a system allows for total control of droplet size, shape, and composition. This approach is utilized to separate, optically detect, and encapsulate two fluorescent analytes from a composite sample mixture. Further to this, we subsequently investigate the potential of the system to be used as a concentration gradient generator through analysis of the segmented analyte bands and droplet composition.
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