Purification, Preliminary Characterization and Hepatoprotective Effects of Polysaccharides from Dandelion Root.
Purification, Preliminary Characterization and Hepatoprotective Effects of Polysaccharides from Dandelion Root.
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DOI：
10.3390/molecules22091409
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发表时间：
2017-08-25
期刊：
影响因子：
--
通讯作者：
Luan L
中科院分区：
文献类型：
--
作者：
Cai L;Wan D;Yi F;Luan L
本研究对葛根水溶性多糖（DRP）的分离纯化、初步鉴定及其保肝作用进行了研究。从DRP中分离得到两种多糖，即DRP 1和DRP 2。两种多糖均为α型多糖，不含蛋白质。DRP 1由葡萄糖、半乳糖和阿拉伯糖组成，分子量为5695 Da; DRP 2由鼠李糖、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖组成，分子量为8882 Da。DRP 1的主链主要由（1→6）-连接-α-d-Glc和（1→ 3，4）-连接-α-d-Glc组成。DRP 2主要由（1→）-连接的-α-d-Ara和（1→）-连接的-α-d-Glc组成。进行了概念验证研究，以评估DRP 1和DRP 2在模拟对乙酰氨基酚（APAP）诱导的人类肝损伤（AILI）的小鼠模型中的治疗潜力。目前的研究表明，DRP 1和DRP 2可以通过激活Nrf 2-Keap 1通路来保护肝脏免受APAP诱导的肝损伤。这些结论表明，DRP 1和DRP 2可能适合作为预防APAP诱导的肝损伤的功能性食品和天然药物。
In this study, purification, preliminary characterization and hepatoprotective effects of water-soluble polysaccharides from dandelion root (DRP) were investigated. Two polysaccharides, DRP1 and DRP2, were isolated from DRP. The two polysaccharides were α-type polysaccharides and didn’t contain protein. DRP1, with a molecular weight of 5695 Da, was composed of glucose, galactose and arabinose, whereas DRP2, with molecular weight of 8882 Da, was composed of rhamnose, galacturonic acid, glucose, galactose and arabinose. The backbone of DRP1 was mainly composed of (1→6)-linked-α-d-Glc and (1→3,4)-linked-α-d-Glc. DRP2 was mainly composed of (1→)-linked-α-d-Ara and (1→)-linked-α-d-Glc. A proof-of-concept study was performed to assess the therapeutic potential of DRP1 and DRP2 in a mouse model that mimics acetaminophen (APAP) -induced liver injury (AILI) in humans. The present study shows DRP1 and DRP2 could protect the liver from APAP-induced hepatic injury by activating the Nrf2-Keap1 pathway. These conclusions demonstrate that the DRP1 and DRP2 might be suitable as functional foods and natural drugs in preventing APAP-induced liver injury.
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影响因子：
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作者：
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影响因子：
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DOI：
10.1042/bj20150568
发表时间：
2015-08-01
期刊：
The Biochemical journal
影响因子：
--
作者：
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