Proteomic profile of culture filtrate from the Brazilian vaccine strain Mycobacterium bovis BCG Moreau compared to M. bovis BCG Pasteur.
Proteomic profile of culture filtrate from the Brazilian vaccine strain Mycobacterium bovis BCG Moreau compared to M. bovis BCG Pasteur.
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DOI：
10.1186/1471-2180-11-80
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发表时间：
2011-04-20
期刊：
影响因子：
4.2
通讯作者：
Mendonça-Lima L
中科院分区：
文献类型：
--
作者：
Berrêdo-Pinho M;Kalume DE;Correa PR;Gomes LH;Pereira MP;da Silva RF;Castello-Branco LR;Degrave WM;Mendonça-Lima L
卡介苗(BCG)是目前唯一可用的结核病(TB)疫苗，由具有基因和表型差异的亚株组成的异质性家族组成。世界卫生组织(WHO)确认，卡介苗亚型在基因组和蛋白质水平上的特征对于更好地理解疫苗至关重要。此外，这些研究有助于开发更有效的结核病疫苗。在这里，我们结合双向电泳(2DE)和质谱仪分析了来自巴西疫苗株BCG Moreau的培养滤液蛋白(CFP)的蛋白质组图谱，并将其与BCG巴斯德菌株的蛋白质组图谱进行了比较。CFPS由于其主要的免疫原性和在发病机制中的作用而被认为是非常重要的，自从早期感染以来，CFPS就可以与宿主细胞相互作用。牛分枝杆菌BCG Moreau CFPs在pH 3-8范围内的2DE蛋白质组图谱允许MS/MS鉴定对应于101种不同蛋白质的158个点，与BCG巴斯德的比较突出了这些BCG菌株之间的巨大相似性。然而，定量分析显示，与BCG巴斯德相比，BCG Moreau中免疫原性蛋白Rv1860(BCG1896，Apa)、Rv1926c(BCG1965c，Mpb63)和Rv1886c(BCG1923c，Ag85B)的表达更高，而一些热休克蛋白，如Rv0440(BCG0479，GroEL2)和Rv0350(BCG0389，DNAK)则表现出相反的模式。在这里，我们报告了来自牛分枝杆菌卡介苗Moreau的CFP的详细的2DE图谱，以及它与卡介苗巴斯德的比较，确定了可能提供疫苗效力相关信息的差异。这些发现有助于对巴西结核病疫苗株的详细描述，揭示了可能有助于更好地理解导致卡介苗对结核病具有不同保护效力的因素的各个方面。
Bacille Calmette-Guerin (BCG) is currently the only available vaccine against tuberculosis (TB) and comprises a heterogeneous family of sub-strains with genotypic and phenotypic differences. The World Health Organization (WHO) affirms that the characterization of BCG sub-strains, both on genomic and proteomic levels, is crucial for a better comprehension of the vaccine. In addition, these studies can contribute in the development of a more efficient vaccine against TB. Here, we combine two-dimensional electrophoresis (2DE) and mass spectrometry to analyse the proteomic profile of culture filtrate proteins (CFPs) from M. bovis BCG Moreau, the Brazilian vaccine strain, comparing it to that of BCG Pasteur. CFPs are considered of great importance given their dominant immunogenicity and role in pathogenesis, being available for interaction with host cells since early infection. The 2DE proteomic map of M. bovis BCG Moreau CFPs in the pH range 3 - 8 allowed the identification of 158 spots corresponding to 101 different proteins, identified by MS/MS. Comparison to BCG Pasteur highlights the great similarity between these BCG strains. However, quantitative analysis shows a higher expression of immunogenic proteins such as Rv1860 (BCG1896, Apa), Rv1926c (BCG1965c, Mpb63) and Rv1886c (BCG1923c, Ag85B) in BCG Moreau when compared to BCG Pasteur, while some heat shock proteins, such as Rv0440 (BCG0479, GroEL2) and Rv0350 (BCG0389, DnaK), show the opposite pattern. Here we report the detailed 2DE profile of CFPs from M. bovis BCG Moreau and its comparison to BCG Pasteur, identifying differences that may provide relevant information on vaccine efficacy. These findings contribute to the detailed characterization of the Brazilian vaccine strain against TB, revealing aspects that may lead to a better understanding of the factors leading to BCG's variable protective efficacy against TB.
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影响因子：
3.1
作者：
Brandt, L;Cunha, JF;Andersen, P
通讯作者：
Andersen, P
影响因子：
3.1
作者：
Ferraz, JC;Stavropoulos, E;Tascon, RE
通讯作者：
Tascon, RE
影响因子：
4.2
作者：
Bendtsen JD;Kiemer L;Fausbøll A;Brunak S
通讯作者：
Brunak S
影响因子：
3.6
作者：
Charlet, D;Mostowy, S;Behr, MA
通讯作者：
Behr, MA
影响因子：
5.5
作者：
Coler, Rhea N.;Dillon, Davin C.;Alderson, Mark R.
通讯作者：
Alderson, Mark R.