The Rice Qa-SNAREs in SYP13 Subfamily Are Involved in Regulating Arbuscular Mycorrhizal Symbiosis and Seed Fertility.
The Rice Qa-SNAREs in SYP13 Subfamily Are Involved in Regulating Arbuscular Mycorrhizal Symbiosis and Seed Fertility.
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SYP13亚科中的水稻Qa-SNARE参与调节丛枝菌根共生和种子肥力
DOI：
10.3389/fpls.2022.898286
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
Qa-SNARE基因SYP 132（亚型α）是影响豆科植物蒺藜苜蓿（Medicago truncatula）丛枝菌根（AM）共生的重要基因。在非豆科植物特别是单子叶植物中，某些SNARE基因是否也参与AM真菌共生尚不清楚。本工作研究了一个在菌根中诱导表达的水稻正基因OsSYP 132和两个不受AM真菌Rhizophagus irregularis诱导表达的旁系同源基因OsSYP 131 a和OsSYP 131 b。在利用CRISPR/Cas9技术产生其突变体后，Ossyp 131 a纯合突变体T0植株表现出矮化表型并且不产生可育种子，表明该基因在种子育性中所需的作用。与豆科植物不同，Ossyp 132突变体表现出正常的菌根表型，Ossyp 131 b突变体也表现出正常的菌根表型。而Ossyp 132双突变体的定殖率和丛枝丰度明显降低，表明真菌在水稻根中的增殖能力减弱。AM标记基因表达水平的降低进一步证实了这种缺陷。因此，我们在水稻中的研究结果表明，虽然SYP 13 II成员表现出特定于共生的进化和诱导模式，但AM共生实际上是由SYP 13 I和SYP 13 II分支的联合作用控制的，揭示了互利共生中SYNTAXIN基因之间的功能冗余。
Qa-SNARE geneSYP132(isoform α) was previously reported to affect arbuscular mycorrhizal (AM) symbiosis in the legume speciesMedicago truncatula. In non-legumes especially monocots, it remains unknown whether certainSNAREgenes are also involved in AM symbiosis. In this work, we studied a rice orthologous geneOsSYP132, which showed induced expression in mycorrhizal roots and two paralogous genesOsSYP131aandOsSYP131b, which were not induced by the AM fungusRhizophagus irregularis. After employing CRISPR/Cas9 technique to generate their mutants, theOssyp131ahomozygous mutant T0plants exhibited a dwarf phenotype and produced no fertile seeds, indicating a required role of this gene in seed fertility. Unlike the case in legume, theOssyp132mutants exhibited normal mycorrhizal phenotype, so did theOssyp131bmutants. In theOssyp131b Ossyp132double mutants, however, the colonization rate and arbuscule abundance level decreased markedly, indicating an impaired fungal proliferation ability in rice roots. Such a defect was further confirmed by the reduced expression levels of AM marker genes. Our results in rice therefore demonstrated that while SYP13II members showed evolutionary and induction patterns specific to symbiosis, AM symbiosis is in fact controlled by the combined action of both SYP13I and SYP13II clades, revealing a functional redundancy among SYNTAXIN genes in mutualism.
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影响因子：
9.4
作者：
Chen, Caiyan;Ane, Jean-Michel;Zhu, Hongyan
通讯作者：
Zhu, Hongyan
DOI：
10.1073/pnas.1200407109
发表时间：
2012-05-22
影响因子：
11.1
作者：
Ivanov, Sergey;Fedorova, Elena E.;Bisseling, Ton
通讯作者：
Bisseling, Ton
DOI：
10.1093/bioinformatics/btu743
发表时间：
2015-04-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Naito Y;Hino K;Bono H;Ui-Tei K
通讯作者：
Ui-Tei K
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
11.6
作者：
Genre, Andrea;Chabaud, Mireille;Bonfante, Paola
通讯作者：
Bonfante, Paola