Sacral neural crest-derived cells enter the aganglionic colon of Ednrb-/- mice along extrinsic nerve fibers.
Sacral neural crest-derived cells enter the aganglionic colon of Ednrb-/- mice along extrinsic nerve fibers.
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DOI：
10.1002/cne.22755
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发表时间：
2012-02-15
影响因子：
2.5
通讯作者：
Epstein, Miles L.
中科院分区：
文献类型：
--
作者：
Erickson, Christopher S.;Zaitoun, Ismail;Haberman, Kathryn M.;Gosain, Ankush;Druckenbrod, Noah R.;Epstein, Miles L.
关键词：
迷走神经和骶骨神经脊细胞都对后肠的肠道神经系统有贡献。由于独立于迷走神经脊外很难观察到骶棘细胞，所以在啮齿类动物中，其对肠道神经系统的贡献的性质和程度还没有很好的确定。为了解决这个问题，我们培育了一些小鼠，在这些小鼠中，末端结肠中只有标记有荧光蛋白的骶骨。我们发现，骶棘细胞与外源性神经纤维相关。我们调查了在无神经节的结肠中发现的外源性神经纤维的来源、出现的时间和特征。我们观察到，盆神经节神经元贡献了许多外源性纤维，这些纤维在环行肌和纵行肌之间的后肠内以及在粘膜下层和浆膜内移动。从胎儿到出生后，沿着这些纤维的骶棘来源细胞的数量逐渐减少。在肌层之间发现了少量的源于骶棘的细胞，表达神经元标志物Hu。我们得出结论，骶棘细胞通过外源性纤维进入后肠，在无神经节准备中，它们在通常由肌间神经节占据的位置形成少量神经元。我们还检查了有神经节的制剂的结肠，发现与外源性神经纤维和新生神经节相关的骶棘来源的细胞。外源性神经纤维是啮齿动物和鸟类的脊骨进入后肠的通道。
Both vagal and sacral neural crest cells contribute to the enteric nervous system in the hindgut. Because it is difficult to visualize sacral crest cells independently of vagal crest, the nature and extent of the sacral crest contribution to the enteric nervous system are not well established in rodents. To overcome this problem we generated mice in which only the fluorescent protein-labeled sacral crest are present in the terminal colon. We found that sacral crest cells were associated with extrinsic nerve fibers. We investigated the source, time of appearance, and characteristics of the extrinsic nerve fibers found in the aganglionic colon. We observed that the pelvic ganglion neurons contributed a number of extrinsic fibers that travel within the hindgut between circular and longitudinal muscles and within the submucosa and serosa. Sacral crest-derived cells along these fibers diminished in number from fetal to post-natal stages. A small number of sacral crest-derived cells were found between the muscle layers and expressed the neuronal marker Hu. We conclude that sacral crest cells enter the hindgut by advancing on extrinsic fibers and, in aganglionic preparations, they form a small number of neurons at sites normally occupied by myenteric ganglia. We also examined the colons of ganglionated preparations and found sacral crest-derived cells associated with both extrinsic nerve fibers and nascent ganglia. Extrinsic nerve fibers serve as a route of entry for both rodent and avian sacral crest into the hindgut.
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影响因子：
3.2
作者：
Herbison, AE;Simonian, SX;Emson, PC
通讯作者：
Emson, PC
影响因子：
2.7
作者：
Burns, AJ;Champeval, D;Le Douarin, NM
通讯作者：
Le Douarin, NM
影响因子：
2.7
作者：
Delalande, Jean-Marie;Barlow, Amanda J.;Bums, Alan J.
通讯作者：
Bums, Alan J.
影响因子：
2.7
作者：
Druckenbrod, NR;Epstein, ML
通讯作者：
Epstein, ML
影响因子：
3.3
作者：
KEAST, JR
通讯作者：
KEAST, JR