Distinct virulent network between healthcare- and community-associated Staphylococcus aureus based on proteomic analysis.
Distinct virulent network between healthcare- and community-associated Staphylococcus aureus based on proteomic analysis.
复制标题
基于蛋白质组学分析的医疗保健和社区相关金黄色葡萄球菌之间独特的毒力网络。
DOI：
10.1186/s12014-017-9178-5
复制
发表时间：
2018
影响因子：
3.8
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
He L;Meng H;Liu Q;Hu M;Wang Y;Chen X;Liu X;Li M
金黄色葡萄球菌(金黄色葡萄球菌或金黄色葡萄球菌)是医疗保健相关(HA)和社区相关(CA)感染的主要原因。HA-SA分离株通常会引起医院内肺炎、血液感染、导尿管相关的尿路感染等。另一方面，CA-SA分离株通常会引起高度致命的疾病，如SSTI和流感后的坏死性出血性肺炎。感染类型的差异部分是由于HA-SA和CA-SA分离株的独特特性所致。例如，HA-SA分离物对宿主上皮细胞表现出很强的粘附性，而CA-SA分离物表现出更高的毒力，这是因为重要的群体感应系统辅助基因调节因子(AGR)的活性增加。因此，这项研究的主要目的是表征HA-SA和CA-SA谱系之间的蛋白质组学差异。本研究利用生物信息学方法构建了蛋白质-蛋白质相互作用网络，并用RT-PCR和Western印迹方法验证了它们的表达。我们用LC-MS/MS证实了AGR系统(agrA和agrC)及其基于蛋白质相互作用网络的相互作用因子(Phop、SrrB、YycG、SarX、SigB和ClpP)在流行的中国CA-SA谱系ST398中的表达显著高于HA-SA谱系ST239。通过RT-PCR进一步验证了这些基因在ST398中的转录上调，提示这些基因/蛋白的高表达可能在CA-SA急性感染中起作用。此外，通过LC-MS/MS分析发现，受AGR系统抑制的表面相关蛋白(FnbpA、SpA、AT1、ClfA、IsaA、ISAB、ltas、SSAA和CnA)在流行的中国HA-SA克隆ST239中的表达显著高于CA-SA克隆ST398。此外，通过Western印迹证实，两种重要的黏附蛋白(At1和ClfA)在ST239中的表达显著增加，这可能与HA-SA的持续感染有关。结果表明，HA-SA和CA-SA的病原学差异至少部分是由于不同的蛋白质组特征造成的。这篇文章的在线版本(10.1186/s12014-0179178-5)载有补充材料，授权用户可以使用。
Staphylococcus aureus (S. aureus or SA) is a leading cause of healthcare-associated (HA-) and community-associated (CA) infection. HA-SA isolates usually cause nosocomial pneumonia, bloodstream infections, catheter-related urinary tract infections, etc. On the other hand, CA-SA isolates usually cause highly fatal diseases, such as SSTIs as well as post influenza necrotic hemorrhagic pneumonia. The differences of the infection types are partially due to the unique characteristics between HA-SA and CA-SA isolates. For example, HA-SA isolates showed strong adherence to host epithelial cells, while CA-SA isolates displayed higher virulence due to the increased activity of the important quorum-sensing system accessory gene regulator (agr). Thus, the aim of this study was to characterize the proteomic difference between HA-SA and CA-SA lineage. In this study, the extracted peptides from those representative strains were analyzed by LC-MS/MS. The protein-protein interaction network was constructed by bioinformatics and their expressions were verified by RT-PCR and Western blot. We demonstrated that Agr system (AgrA and AgrC) and its interactive factors (PhoP, SrrB, YycG, SarX, SigB and ClpP) based on the protein–protein interaction network were expressed significantly higher in the epidemic Chinese CA-SA lineage ST398 compared to HA-SA lineage ST239 by LC-MS/MS. We further verified the increased transcription of all these genes in ST398 by RT-PCR, suggesting that the higher expression of these genes/proteins probably play role in the acute infection of CA-SA. Moreover, surface-related proteins (FnbpA, SpA, Atl, ClfA, IsaA, IsaB, LtaS, SsaA and Cna) that are repressed by the Agr system have significantly higher expression in the epidemic Chinese HA-SA clone ST239 in comparison to CA-SA lineage ST398 by LC-MS/MS. Furthermore, we confirmed the significantly increased expression of two important adhesive proteins (Atl and ClfA) in ST239 by Western blot, which may contribute to the durative infection of HA-SA. The results suggest that the different proteomic profile, at least partially, contribute to the pathogenic differences between HA-SA and CA-SA. The online version of this article (10.1186/s12014-017-9178-5) contains supplementary material, which is available to authorized users.
登录
查看更多内容
与氟喹诺酮类抗性相关的不同适应性会影响各种多耐药细菌的克隆动力学。
影响因子：
5.2
作者：
Fuzi M
通讯作者：
Fuzi M
影响因子：
6.7
作者：
Edwards AM;Potts JR;Josefsson E;Massey RC
通讯作者：
Massey RC
影响因子：
168.9
作者：
DeLeo, Frank R.;Otto, Michael;Kreiswirth, Barry N.;Chambers, Henry F.
通讯作者：
Chambers, Henry F.
影响因子：
4.4
作者：
Jones, Marcus B.;Montgomery, Christopher P.;Daum, Robert S.
通讯作者：
Daum, Robert S.
影响因子：
7
作者：
Holden MT;Hsu LY;Kurt K;Weinert LA;Mather AE;Harris SR;Strommenger B;Layer F;Witte W;de Lencastre H;Skov R;Westh H;Zemlicková H;Coombs G;Kearns AM;Hill RL;Edgeworth J;Gould I;Gant V;Cooke J;Edwards GF;McAdam PR;Templeton KE;McCann A;Zhou Z;Castillo-Ramírez S;Feil EJ;Hudson LO;Enright MC;Balloux F;Aanensen DM;Spratt BG;Fitzgerald JR;Parkhill J;Achtman M;Bentley SD;Nübel U
通讯作者：
Nübel U