T follicular regulatory cells infiltrate the human airways during the onset of acute respiratory distress syndrome and regulate the development of B regulatory cells.
T follicular regulatory cells infiltrate the human airways during the onset of acute respiratory distress syndrome and regulate the development of B regulatory cells.
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急性呼吸窘迫综合征发作期间，滤泡 T 调节细胞浸润人体气道并调节 B 调节细胞的发育
DOI：
10.1007/s12026-018-9014-7
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发表时间：
2018-08
影响因子：
4.4
通讯作者：
Xu S
中科院分区：
文献类型：
--
作者：
Li H;Zhou R;Wang C;Li Y;Zheng G;Jiang S;Dong T;Bai J;Xu S
滤泡调节性T细胞（Tfr）是调节性T细胞（Treg）的CXCR 5 + Foxp 3+亚群，在调节生殖中心反应和调节淋巴结中的免疫环境中具有关键作用。研究表明，Tfr细胞的比例可能在急性炎症期间增加。本研究旨在探讨Tfr细胞在急性呼吸窘迫综合征（ARDS）中的作用。我们发现，Tfr细胞显着丰富的外周血和微型支气管肺泡灌洗（BAL）在ARDS发病期间。值得注意的是，Tfr细胞代表了mini-BAL样品中的大多数Treg细胞。Tfr细胞也显示CTLA-4、IL-10和TGF-β表达，但与非Tfr Treg细胞相比，Tfr细胞的CTLA-4和IL-10表达轻微降低。Tfr细胞和非Tfr Treg细胞均抑制自体CD 4 + CD 25 −T细胞的增殖;然而，Tfr细胞显示出略微降低的抑制能力。随后，将B细胞与自体Tfr细胞或非Tfr Treg细胞共孵育。有趣的是，我们发现在与Tfr细胞一起孵育后IL-10+布雷格细胞的频率显著高于与非Tfr Treg细胞一起孵育，这表明Tfr细胞在诱导IL-10+布雷格细胞方面更有效。总之，这些结果表明Tfr细胞是Treg细胞的相似但独特的亚群。由于Tfr细胞在ARDS患者中，特别是在肺浸润中强烈富集，它们可能在ARDS中发挥关键的改善作用。
T follicular regulatory (Tfr) cell is a CXCR5+Foxp3+subset of T regulatory (Treg) cell with critical roles in regulating germinal center responses and modulating the immune environment in the lymph nodes. Studies have shown that the proportion of Tfr cells may increase during acute inflammation. In this study, we investigated the role of Tfr cells in acute respiratory distress syndrome (ARDS). We found that Tfr cells were significantly enriched in peripheral blood and in mini-bronchoalveolar lavage (BAL) during the onset of ARDS. Notably, Tfr cells represented the majority of Treg cells in the mini-BAL samples. Tfr cells also showed CTLA-4, IL-10, and TGF-β expression, but compared to the non-Tfr Treg cells, the CTLA-4 and IL-10 expression by Tfr cells were slightly reduced. Both Tfr cells and non-Tfr Treg cells suppressed the proliferation of autologous CD4+CD25−T cells; however, the Tfr cells displayed slightly reduced suppression capacity. Subsequently, B cells were co-incubated with autologous Tfr cells or non-Tfr Treg cells. Interestingly, we found that the frequency of IL-10+Breg cells was significantly higher following incubation with Tfr cells than with non-Tfr Treg cells, which suggested that Tfr cells were more potent at inducing IL-10+Breg cells. Together, these results demonstrated that Tfr cells were a similar but distinctive subset of Treg cells. Given that Tfr cells were strongly enriched in ARDS patients, especially in the lung infiltrates, they may exert critical ameliorating effects in ARDS.
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影响因子：
4.4
作者：
Wollenberg, Ivonne;Agua-Doce, Ana;Graca, Luis
通讯作者：
Graca, Luis
影响因子：
5.1
作者：
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影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
Pesenti, Antonio